Slim defines a novel family of LIM-proteins expressed in skeletal muscle
Slim defines a novel family of LIM-proteins expressed in skeletal muscle
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DOI：
10.1006/bbrc.1996.1222
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发表时间：
1996-08-14
影响因子：
3.1
通讯作者：
Madgwick, AJA
中科院分区：
文献类型：
--
作者：
Morgan, MJ;Madgwick, AJA
我们通过对重叠的cDNA序列进行比对，组装了骨骼肌LIM-Protein SLIM的完整蛋白质序列。这些cDNA序列是从我们自己的测序和BLASTn搜索的非冗余cDNA数据库中鉴定出来的。预测的SILM蛋白序列包括4个LIM结构域和位于N-末端的新的单个锌指结构域。鉴定了与SLIM相似的序列，并将其命名为SLIM2和Slim3。Slim3cDNA序列被鉴定为LIM-蛋白质序列的部分序列，LIM结构域的数目和间距与所有三种蛋白质序列相同。Northern分析检测到人咬肌RNA中每种蛋白的mRNA表达。我们认为这些蛋白属于在人类骨骼肌中表达的LIM蛋白家族，(C)1996学术出版社，Inc.。
We have assembled the complete protein sequence of the skeletal muscle LIM-protein SLIM by aligning overlapping cDNA sequences. These cDNA sequences were identified from our own sequencing and from BLASTn searches of non-redundant cDNA databases. The predicted SLIM protein sequence included four LIM-domains and a novel single zinc finger domain located in the N-terminal region. Similar sequences to SLIM were identified and termed SLIM2 and SLIM3. The SLIM3 cDNA sequence was identified subsequently as a partial sequence of the of the LIM-protein DRAL, The number and spacing of the LIM domains was common to all three protein sequences. The mRNA for each protein was detected in human masseter muscle RNA by Northern analysis. We suggest that these proteins belong to a novel family of LIM proteins that are expressed in human skeletal muscle, (C) 1996 Academic Press, Inc.