Biological effect of epidermal growth factor on the in vitro growth of human tumors.
Biological effect of epidermal growth factor on the in vitro growth of human tumors.
复制标题
表皮生长因子对人类肿瘤体外生长的生物学作用。
作者：
S. Singletary;F. Baker;G. Spitzer;S. Tucker;B. Tomasovic;W. Brock;J. Ajani;Kelly Am
在含有转铁蛋白、胰岛素、氢化可的松和雌二醇的细胞黏附基质人肿瘤培养体系中，观察了表皮生长因子(EGF)对186例人恶性肿瘤标本(黑色素瘤45例、肉瘤32例、肺癌56例、妇科16例、乳腺癌14例、泌尿生殖系12例、胃肠道癌11例)体外生长的影响。EGF使186个肿瘤中81%的肿瘤生长至少50%，54%的肿瘤生长超过100%。EGF诱导的生长增强与细胞分裂加速有关，与肿瘤类型无关，而与克隆形成单位的实际数量增加无关。实现90%肿瘤细胞杀伤率所需的细胞周期非依赖性阿霉素和顺铂的药物浓度不受肿瘤对EGF的反应性的影响。
The effect of epidermal growth factor (EGF) on the in vitro growth of 186 malignant human tumor specimens (45 melanomas, 32 sarcomas, and 56 lung, 16 gynecological, 14 breast, 12 genitourinary, and 11 gastrointestinal carcinomas) was evaluated in the cellular adhesive matrix human tumor culture system supplemented with transferrin, insulin, hydrocortisone, and estradiol. EGF increased tumor growth by at least 50% in 81% of the 186 tumors and by over 100% in 54%. The enhanced growth induced by EGF was related to an accelerated cellular division independent of tumor type and not to an increase in the actual number of clonogenic units. The drug concentrations of cell cycle-independent Adriamycin and cisplatin needed to achieve a 90% tumor cell kill were not altered by the responsiveness of the tumor to EGF.