"Neighbourhood watch" model: embryonic epiblast cells assess positional information in relation to their neighbours
"Neighbourhood watch" model: embryonic epiblast cells assess positional information in relation to their neighbours
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“邻里监视”模型：胚胎外胚层细胞评估与其邻居相关的位置信息
DOI：
10.1101/2021.04.22.440894
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Lee H
中科院分区：
文献类型：
--
作者：
Lee H
在许多发育和再生系统中，组织模式是通过信息形态发生素的梯度建立的，但我们对细胞如何解释这些知之甚少。使用早期鸡胚的实验操作，包括诱导剂（VG1或ACTIVIN）和抑制剂（BMP 4）的错误表达，我们测试了两种替代模型，以解释原始条纹形成的位点相对于胚胎的其余部分是如何定位的。在一个模型中，细胞自主地读取形态原浓度。在另一种情况下，细胞感知相对于其邻近区域的形态发生状态的变化。我们发现，只有后者的模型可以解释实验结果，包括一些反直觉的预测。这种机制（我们称之为“邻里守望”模型）阐明了经典的“法国国旗问题”，以及位置信息是如何被一张细胞在一个大的发展系统解释。
In many developing and regenerating systems, tissue pattern is established through gradients of informative morphogens, but we know little about how cells interpret these. Using experimental manipulation of early chick embryos, including misexpression of an inducer (VG1 or ACTIVIN) and an inhibitor (BMP4), we test two alternative models for their ability to explain how the site of primitive streak formation is positioned relative to the rest of the embryo. In one model, cells read morphogen concentrations cell-autonomously. In the other, cells sense changes in morphogen status relative to their neighbourhood. We find that only the latter model can account for the experimental results, including some counter-intuitive predictions. This mechanism (which we name the ‘neighbourhood watch’ model) illuminates the classic ‘French Flag Problem’ and how positional information is interpreted by a sheet of cells in a large developing system.