Identification of the spinal neural network involved in coordination of micturition in the male cat.
Identification of the spinal neural network involved in coordination of micturition in the male cat.
复制标题
鉴定参与雄性猫排尿协调的脊髓神经网络。
DOI：
10.1016/s0006-8993(98)00340-0
复制
发表时间：
1998
期刊：
影响因子：
2.9
通讯作者：
Haxhiu,MA
中科院分区：
文献类型：
--
作者：
Grill,WM;Wang,B;Hadziefendic,S;Haxhiu,MA
在这些研究中，我们使用Fos蛋白的表达来识别脊髓内调节雄性猫排尿的细胞。编码Fos蛋白的即刻早期基因c-fos可以通过增加电活动在神经元中快速而瞬时地诱导。动物用α-氯醛糖麻醉，接受四种刺激方案中的一种：电刺激盆神经、电刺激阴部神经、等长排尿时间(通过结扎近端尿路并将生理盐水注入膀胱)或电刺激巴林顿核。刺激结束后，灌流动物，用免疫细胞化学方法显示表达Fos样免疫反应(FLI)的神经元。与手术但未受刺激的对照组相比，每种方案的刺激均可导致大量的Fos阳性神经元表达，仅有少量Fos阳性神经元定位于背角浅层。在等长排尿或刺激Barrington核的动物中，在双侧脊髓(S1-S3)可见表达FLI的神经元，并定位于背角浅层的外侧部分(板层I和II)、中间外侧区(板层V-VII)和中央管周围(板层X和内侧板V-VII)。中间外侧区域似乎包含两组细胞：一组大的多极细胞和一组小的圆形细胞。在浅背角内侧仅见少量Fos免疫反应阳性核，腹角神经元未见Fos免疫反应阳性神经元。电刺激阴部或盆腔神经导致显示FLI的细胞数量较少，空间分布较少。这些结果确定了在排尿周期中活跃的脊髓神经元，并表明与行为相关的刺激(等长排尿)比重复电刺激组成的周围神经产生更广泛和更强的Fos表达。
In these studies, we used the expression of Fos protein to identify cells within the spinal cord that regulate micturition in male cats. The immediate early gene c-fos that encodes the Fos protein can be induced rapidly and transiently in neurons by increased electrical activity. Animals were anesthetized with alpha-chloralose, and received one of four stimulus protocols: electrical stimulation of the pelvic nerve, electrical stimulation of the pudendal nerve, a period of isometric micturition (induced by ligating the proximal urethra and infusing saline into the bladder), or electrical stimulation of Barrington's nucleus. After the period of stimulation, the animals were perfused and neurons expressing Fos-like immunoreactivity (FLI) were visualized with immunocytochemical methods. Stimulation with each protocol resulted in a substantially larger number of neurons expressing FLI than in operated but unstimulated controls, which exhibited few Fos-positive neurons localized to the superficial dorsal horn. In animals undergoing isometric micturition or stimulation of Barrington's nucleus, neurons exhibiting FLI were found bilaterally in the sacral (S1–S3) spinal cord and were localized to the lateral portion of the superficial dorsal horn (laminae I and II), in the intermediolateral region (lateral laminae V–VII), and around the central canal (lamina X and medial laminae V–VII). The intermediolateral region appeared to contain two populations of cells exhibiting FLI: a group of large multipolar cells and a group of small round cells. Few Fos-immunoreactive nuclei were observed in the medial portion of the superficial dorsal horn, and FLI was not observed in ventral horn neurons. Electrical stimulation of the pudendal or pelvic nerves resulted in fewer numbers of cells exhibiting FLI, with a less widespread spatial distribution. These results identify spinal neurons that are active during the micturition cycle, and demonstrate that a behaviorally relevant stimulus (isometric micturition) generated more widespread and greater intensity of Fos expression than repetitive electrical stimulation of the component peripheral nerves.