Optimal staining methods for delineation of cortical areas and neuron counts in human brains.
Optimal staining methods for delineation of cortical areas and neuron counts in human brains.
复制标题
用于描绘人脑皮质区域和神经元计数的最佳染色方法。
DOI：
10.1006/nimg.1999.0417
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Rajkowska,G
中科院分区：
文献类型：
--
作者：
Uylings,HB;Zilles,K;Rajkowska,G
对于人脑皮质区域的细胞构筑描绘，由于胞体和神经纤维之间的强烈对比，对于体细胞的Galyas染色比经典的Nissl染色更可取，当使用图像分析系统时尤其如此。然而，由于小神经元和神经胶质细胞之间缺乏明显的细胞学特征，这种Galyas染色似乎不适合于计算相关脑区的神经元数量。对于细胞计数，Nissl更可取。在细胞计数的最佳设计中，至少必须同时应用Galyas染色和Nissl染色，前者用于皮质区域的细胞构筑描绘，后者用于计算相关皮质区域的神经元数量。
For cytoarchitectonic delineation of cortical areas in human brain, the Gallyas staining for somata with its sharp contrast between cell bodies and neuropil is preferable to the classical Nissl staining, the more so when an image analysis system is used. This Gallyas staining, however, does not appear to be appropriate for counting neuron numbers in pertinent brain areas, due to the lack of distinct cytological features between small neurons and glial cells. For cell counting Nissl is preferable. In an optimal design for cell counting at least both the Gallyas and the Nissl staining must be applied, the former staining for cytoarchitectural delineaton of cortical areas and the latter for counting the number of neurons in the pertinent cortical areas.