Obtaining autotetraploids in vitro at a high frequency in Citrus sinensis
Obtaining autotetraploids in vitro at a high frequency in Citrus sinensis
复制标题
DOI：
10.1007/s11240-007-9240-5
复制
发表时间：
2007-06
期刊：
影响因子：
--
通讯作者：
Jun-e Zhang;Min Zhang;X. Deng
中科院分区：
文献类型：
--
作者：
Jun-e Zhang;Min Zhang;X. Deng
四倍体植株是倍体间杂交创造三倍体无核柑橘所必需的。本文报道了一种简单有效的甜橙子同源四倍体离体培养方法。使用流式细胞术分析“安柳城”甜橙子愈伤组织中的细胞分裂活性，以确定细胞分裂的峰值频率，此时液体培养基和固体培养基中的愈伤组织用1000 mg l− 1秋水仙素处理。液体培养基和固体培养基中DNA含量变异细胞的比例分别从11.0%提高到44.4%和59.0%。从处理的愈伤组织再生的47株植株中，通过胚状体发生共回收到20株四倍体植株。同源四倍体均来源于不同的胚状体。同源四倍体将是有用的亲本进行倍体间杂交，以产生有商业价值的无核三倍体柑橘品种。
Tetraploid plants are essential for interploid hybridization to create triploid seedless citrus. Here we report a simple and efficient in vitro method for generating autotetraploids for sweet orange (Citrus sinensis). Cell-division activity in ‘Anliucheng’ sweet orange callus was analyzed using flow cytometry to determine the peak frequency of cell division at which time callus in a liquid media and solid media was treated with 1000 mg l−1colchicine. The percentage of the DNA-content-varied cells in the callus increased markedly from 11.0% to 44.4% and to 59.0% for liquid and solid media respectively. A total of 20 tetraploid plantlets were recovered via embryogenesis from 47 plantlets regenerated from the treated callus. All the autotetraploids were derived from different embryoids. Autotetraploids will be useful parents for interploid hybridization to generate commercially valuable seedless triploid citrus cultivars.