Comparison of the genome structure of the self-incompatibility (S) locus in interspecific pairs of S haplotypes
Comparison of the genome structure of the self-incompatibility (S) locus in interspecific pairs of S haplotypes
复制标题
DOI：
10.1534/genetics.104.037267
复制
发表时间：
2006-06-01
期刊：
影响因子：
3.3
通讯作者：
Nishio, Takeshi
中科院分区：
文献类型：
--
作者：
Fujimoto, Ryo;Okazaki, Keiichi;Nishio, Takeshi
芸苔属植物自交不亲和性识别特异性的决定因素是柱头中的SRK和花粉中的SP11/SCR，这两个基因都由S基因座编码。许多SRK和SP 11/SCR等位基因的核苷酸序列分析已经鉴定了几对种间S单倍型，它们在B之间具有高度相似的序列。oleracea和B.拉帕。这些种间的S单倍型被认为是来自共同的祖先，并保持了相同的识别特异性后形成。本研究通过对SRK、SP11/SCR及其侧翼序列的测定，比较了3对种间S单倍型的基因组结构。B中SRK和SP 11/SCR之间的区域。结果表明，甘蓝的叶片比B的叶片长得多。在B的S基因座中鉴定出rapa和几个反转录转座子样序列。甘蓝属植物在7个逆转录转座子样序列中，发现6个序列属于ty 3吉普赛组。逆转录转座子样序列的gag序列在遗传学上彼此不同。在使用逆转录转座子样序列作为探针的Southern印迹分析中，B.甘蓝基因组比B基因组显示出更多的信号。rapa基因组做到了。这些研究结果表明，在自交不亲和植物物种的S位点和基因组进化的作用。
The determinants of recognition specificity of self-incompatibility in Brassica are SRK in the stigma and SP11/SCR in the pollen, both of which are encoded in the S locus. The nucleotide sequence analyses of many SRK and SP11/SCR alleles have identified several interspecific pairs of S haplotypes having highly similar sequences between B. oleracea and B. rapa. These interspecific pairs of S haplotypes are considered to be derived from common ancestors and to have maintained the same recognition specificity after speciation. In this study, the genome structures of three interspecific pairs of S haplotypes were compared by sequencing SRK, SP11/SCR and their flanking regions. Regions between SRK and SP11/SCR in B. oleracea were demonstrated to be much longer than those of B. rapa and several retrotransposon-like sequences were identified in the S locus in B. oleracea. Among the seven retrotransposon-like sequences, six sequences were found to belong to the ty3 gypsy group. The gag sequences of the retrotransposon-like sequences were phylogenetically different from each other. In Southern blot analysis using retrotransposon-like sequences as probes, the B. oleracea genome showed more signals than the B. rapa genome did. These findings suggest a role for the S locus and genome evolution in self-incompatible plant species.