Fibroblast adhesion to RGDS shows novel features compared with fibronectin.
Fibroblast adhesion to RGDS shows novel features compared with fibronectin.
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DOI：
10.1083/jcb.105.1.507
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发表时间：
1987-07
影响因子：
7.8
通讯作者：
Rees, D A
中科院分区：
文献类型：
--
作者：
Streeter, H B;Rees, D A
如之前其他人所示，纤连蛋白的成纤维细胞附着和扩散活性被来自细胞结合结构域的短肽（RGDS或更长）模拟。正常大鼠肾成纤维细胞表现出类似的附着动力学肽GRGDSC或牛血浆纤连蛋白和结合到任何基质被单独肽抑制。然而，我们现在证明，肽和纤连蛋白之间的生物活性有相当大的差异。特别是，细胞在肽包被的基质上形成了新的粘附结构。干涉反射显微镜检查显示粘附膜的小圆形深灰色/黑色斑块（“斑点”）占优势，具有相对较少的局部粘附，其仅发生在最外层细胞边缘，与其在纤连蛋白上散布的细胞中的分布相反。这些斑点对去污剂提取具有抗性，粘着斑蛋白染色不太强烈或根本不染色。电子显微镜在垂直薄切片显示，腹侧表面的细胞的特点是由“点接触”，相应的大小的斑点结构所看到的干涉反射显微镜，只有偶尔与微丝。细胞还需要比纤连蛋白更高的肽基质负载来促进扩散，并且继续扩散得不太快，程度也较小，形成非常少和非常细的肌动蛋白电缆。
As previously shown by others, the fibroblast attachment and spreading activity of fibronectin is mimicked by a short peptide (RGDS or longer) from the cell binding domain. Normal rat kidney fibroblasts showed similar attachment kinetics on either peptide GRGDSC or bovine plasma fibronectin and binding to either substratum was inhibited by peptide alone. We now demonstrate, however, considerable differences in biological activity between peptide and fibronectin. In particular, cells developed novel adhesion structures on peptide-coated substrata. Interference reflection microscopy showed a predominance of small round dark grey/black patches of adherent membrane ("spots") with relatively few focal adhesions, which occurred only at the outermost cell margins in contrast to their distribution in cells spread on fibronectin. The spots were resistant to detergent extraction and stained less strongly or not at all for vinculin. Electron microscopy in vertical thin section showed that the ventral surface of the cell was characterized by "point-contacts", corresponding in size to the spot structures seen by interference reflection microscopy, and which were only occasionally associated with microfilaments. Cells also required a higher substratum loading of peptide than fibronectin to promote spreading and proceeded to spread less rapidly and to a lesser extent, developing very few and extremely fine actin cables.