Silicon-on-Insulator microring resonator for sensitive and label-free biosensing
Silicon-on-Insulator microring resonator for sensitive and label-free biosensing
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DOI：
10.1364/oe.15.007610
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发表时间：
2007-06-11
期刊：
影响因子：
3.8
通讯作者：
Baets, Roel
中科院分区：
文献类型：
--
作者：
De Vos, Katrien;Bartolozzi, Irene;Baets, Roel
无标记生物传感器试图克服生物传感器依赖于标记分子检测的稳定性和可靠性问题。我们提出了一种基于绝缘体上硅（SOI）微环腔的无标签生物传感器，适用于小于10x10 μ m(2)的区域。谐振波长的变化发生时，一个腔的环境发生变化，用于传感。虽然理论上对体折射率变化的性能适中（10(-5)），但由于其极小的尺寸，该器件在绝对分子质量传感（理论灵敏度为1fg分子质量）方面表现出色。我们使用亲和素/生物素高亲和偶联证明了良好的重复性和检测蛋白浓度低至10ng/ml。采用深紫外光刻技术制造，可以实现廉价的批量生产，并与完整的芯片上实验室设备的电子功能集成。(c) 2007年美国光学学会。
Label-free biosensors attempt to overcome the stability and reliability problems of biosensors relying on the detection of labeled molecules. We propose a label-free biosensor based on microring cavities in Silicon-on-Insulator (SOI) that fits in an area below 10x10 mu m(2). The resonance wavelength shift that occurs when the surroundings of a cavity is changed, is used for sensing. While theoretically the performance for bulk refractive index changes is moderate (10(-5)), this device performs outstanding in terms of absolute molecular mass sensing (theoretical sensitivity of 1fg molecular mass) thanks to its extremely small dimensions. We use the avidin/biotin high affinity couple to demonstrate good repeatability and detection of protein concentrations down to 10ng/ml. Fabrication with Deep UV lithography allows for cheap mass production and integration with electronic functions for complete lab-on-chip devices. (c) 2007 Optical Society of America.