Metabolomic association analysis reveals defense related secondary metabolic reprogramming in callus of Scutellaria baicalensis Georgi induced by endophytic Pseudomonas sp. 2B
Metabolomic association analysis reveals defense related secondary metabolic reprogramming in callus of Scutellaria baicalensis Georgi induced by endophytic Pseudomonas sp. 2B
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DOI：
10.1016/j.indcrop.2022.115409
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发表时间：
2022
影响因子：
5.9
通讯作者：
Jingyuan Xu;Ruoyun Shi;Yijie Cheng;Guoyong Xie;Yan Zhu;Minjian Qin
中科院分区：
文献类型：
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作者：
Jingyuan Xu;Ruoyun Shi;Yijie Cheng;Guoyong Xie;Yan Zhu;Minjian Qin
植物与内生菌的相互作用可以影响寄主植物的次生代谢。黄芩是一种常用中药，在我国广泛栽培。到目前为止，内生菌对S. baicalensisis仍不清楚。为了解决这一问题，本研究采用非靶向代谢组学方法，以黄芩愈伤组织和内生假单胞菌2B为材料，研究了黄芩次生代谢的总体变化。进一步检测了多酚氧化酶（PPO）活性和类黄酮生物合成途径中关键基因的表达模式。通过非靶向代谢组学分析，共发现1814个差异代谢产物，鉴定出的差异代谢产物主要为黄酮类、蒽醌类、木质素类和苯乙醇类。目标定量分析表明，黄芩苷和汉黄芩苷在愈伤组织中的含量降低。黄芩素、汉黄芩素和白杨素的积累均受假单胞菌2B的影响。PPO活性在此过程中也有所提高。此外，在内生菌-愈伤组织的双重培养中，黄酮合酶（sbFNS II-2）和查耳酮合酶（sbCHS 2）的转录本显著降低。肉桂酸特异性辅酶A连接酶（sbCLL 7）和UDP-葡萄糖醛酸黄芩素7-O-葡萄糖醛酸转移酶（sbUBGAT）转录本的表达得到促进。结果表明，内生假单胞菌2B对黄芩防御相关的次生代谢具有重编程作用，在黄芩的生物防治中具有潜在的应用价值。黄芩
Interactions between plants and endophyte can influence the secondary metabolism in host plants. Scutellaria baicalensis Georgi, is widely used as traditional Chinese medicine and cultivated extensively in China. Until now, the influence of endophytes on secondary metabolism ofS. baicalensisis still unclear. To address this issue, non-targeted metabolomics tool was performed to explore the global secondary metabolic changes inS. baicalensiswith a dual culture of callus and endophytic Pseudomonassp.2B. Further, the activity of polyphenol oxidase (PPO) and the expression patterns of key genes in flavonoid biosynthesis pathway were detected. Through the analysis of non-targeted metabolomics, a total of 1814 differential metabolites were found and the identified differential metabolites were mainly belonged to flavonoids, anthraquinones, lignin and phenylethanoids. The target quantitative analysis revealed that the contents of baicalin and wogonoside decreased in callus ofS. baicalensisco-cultured with Pseudomonassp.2B, but the accumulations of baicalein, wogonin and chrysin were promoted. The activity of PPO was also increased in this process. Furthermore, there was a significant decrease in flavone synthase (sbFNS II-2) and chalcone synthase (sbCHS2) transcripts in dual culture of endophyte-callus. The expression of cinnamic acid–specific coenzyme A ligase (sbCLL7) and the UDP-glucuronate baicalein 7-O-glucuronosyltransferase (sbUBGAT) transcripts were promoted. The results demonstrated that endophyticPseudomonas sp.2B could reprogram defense related secondary metabolism inS. baicalensisand had a potential application value in biocontrol for cultivation ofS. baicalensis.