Developmental and androgenic regulation of the immunocytochemical distribution of mK1, a true tissue kallikrein, in the granular convoluted tubule of the mouse submandibular gland.
Developmental and androgenic regulation of the immunocytochemical distribution of mK1, a true tissue kallikrein, in the granular convoluted tubule of the mouse submandibular gland.
复制标题
mK1（一种真正的组织激肽释放酶）在小鼠颌下腺颗粒曲管中的免疫细胞化学分布的发育和雄激素调节。
DOI：
10.1177/002215540205000202
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Gresik,EdwardW
中科院分区：
文献类型：
--
作者：
Kurabuchi,Shingo;Hosoi,Kazuo;Gresik,EdwardW
用间接酶标法和免疫金标记抗体法，分别在光镜和电子显微镜下观察了雄激素对发育和成年小鼠颌下腺(SMG)内真组织激肽释放酶MK1免疫细胞化学分布的影响。3周龄时，未成熟的颗粒曲管(GCT)细胞几乎全部免疫染色。在4周龄(青春期开始)，GCTS出现了雌雄不同的形态差异，其中一些细胞免疫阴性。此后，男性SMG中免疫阴性的GCT细胞比女性更丰富，Mk1的染色强度在细胞间有相当大的差异，尤其是在男性。仅在男性GCT节段可见少量细长的GCT细胞，呈强阳性反应。雄性去势导致GCT免疫阳性细胞数量增加，而双氢睾酮(DHT)处理则减少了两性SMG中GCT免疫阳性细胞的数量。用DHT治疗6天的女性，可见细长的GCT细胞表达Mk1。而雌性SMG注射DHT 2周后，未见细长GCT细胞。免疫电子显微镜显示，在雄性SMGs中，这种细胞与青春期前小鼠的未成熟GCT细胞非常相似，有少量均匀标记金粒的小分泌颗粒，稀疏的高尔基体和粗面内质网，以及基底部的褶皱。在成熟的雄性SMG以及DHT处理的雌性和去势雄性的SMG中，典型的GCT细胞具有发达的高尔基体和网状的RER，但很少或没有基底部褶皱，而在女性腺体中，相应的细胞具有中等发达的RER和一些基底部褶皱。这些结果表明，Mk1是未成熟GCT细胞特有的一种酶，其合成部分受到雄激素的抑制，导致免疫阳性细胞数量减少。
The action of androgens on the immunocytochemical distribution of mK1, a true tissue kallikrein, was examined in the submandibular gland (SMG) of developing and adult mice by indirect enzyme-labeled and immunogold-labeled antibody methods for light and electron microscopy, respectively. In both sexes at 3 weeks of age, essentially all of the immature granular convoluted tubule (GCT) cells were uniformly immunostained. At 4 weeks of age (the onset of puberty), morphological differences between the two sexes appeared in the GCTs, in which some cells became immunonegative. Thereafter, the immunonegative GCT cells became more abundant in the SMG of males than of females and considerable intercellular variation in staining intensity for mK1 was seen, especially in males. A few slender GCT cells with strong immunoreactivity appeared in GCT segments only in males. Castration of males resulted in an increase in the number of immunopositive GCT cells, whereas administration of dihydrotestosterone (DHT) decreased the number of immunopositive GCT cells in the SMGs of both sexes. Slender GCT cells immunoreactive for mK1 were seen in females treated with DHT for 6 days. However, there were no immunostained slender GCT cells in female SMGs after injection of DHT for 2 weeks. Immuno-electron microscopy disclosed this type of cell in male SMGs, which closely resembles immature GCT cells of prepubertal mice, with a few small secretory granules uniformly labeled with gold particles, a sparse Golgi apparatus and RER, and basal infoldings. In mature male SMGs and in SMGs of DHT-treated females and castrated males, typical GCT cells had a well-developed Golgi apparatus and a net-like RER but few to no basal infoldings, whereas in the female gland equivalent cells had moderately developed RER and some basal infoldings. These results suggest that mK1 is one of the enzymes characteristically present in immature GCT cells and that its synthesis is inhibited in part by androgens, resulting in decreased numbers of immunopositive cells.