Comparative and quantitative investigation of cell labeling of a 12-nm DMSA-coated Fe_3O_4 magnetic nanoparticle with multiple mammalian cell lines
Comparative and quantitative investigation of cell labeling of a 12-nm DMSA-coated Fe_3O_4 magnetic nanoparticle with multiple mammalian cell lines
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DOI：
10.1557/jmr.2010.60
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发表时间：
2011-03
影响因子：
2.7
通讯作者：
Yingxun Liu;Jinke Wang
中科院分区：
文献类型：
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作者：
Yingxun Liu;Jinke Wang
本文研究了12 nm内消旋-2，3-二巯基丁二酸（DMSA）修饰的Fe_3O_4磁性纳米粒子对多种哺乳动物细胞的标记。用不同浓度的纳米颗粒处理六种不同的细胞，包括RAW 264.7、Hepa 1 -6、THP-1、HepG 2、HeLa和HL-7702（20~100 μg/mL）作用不同时间（2~72 h），并通过普鲁士蓝染色观察纳米颗粒的细胞内内化和用荧光法测量相应的细胞铁负荷来评价标记效果。比色分析结果表明，纳米颗粒可以标记所有研究的细胞。然而，不同细胞之间的标记效率并不相同，这取决于细胞类型、纳米颗粒的浓度以及用纳米颗粒处理细胞的时间。相比之下，RAW264.7在任何浓度的纳米颗粒下都比其他细胞更有效地标记。RAW264.7的铁负载量随着纳米颗粒的浓度和处理时间的增加而显著增加。然而，两种人肝细胞（HepG 2和HL-7720）都标记有最低的铁负载。细胞活力的测量显示，在常见的铁纳米颗粒的体内应用剂量（30 μg/mL）下，所有细胞的生长不受纳米颗粒的影响，证明纳米颗粒具有更好的生物相容性。
This work investigated the cell labeling of 12-nm meso-2,-3-dimercaptosuccinic acid (DMSA)-coated Fe_3O_4 magnetic nanoparticles with multiple mammalian cells. Six different cells, including RAW264.7, Hepa1-6, THP-1, HepG2, HeLa, and HL-7702, were treated with the nanoparticles at various concentrations (20~100 μg/mL) for different times (2~72 h), and the labeling effect was evaluated by observing the intracellular internalization of the nanoparticles with Prussian blue staining and measuring the corresponding cellular iron loading with colorimetric assay. The results demonstrated that the nanoparticles could label all cells studied. However, the labeling efficiency was not the same between different cells, which depended on the cell types, the nanoparticles’ concentration, and the time of treating cells with the nanoparticles. In comparison, RAW264.7 was labeled more effectively than other cells at any concentration of the nanoparticles. The iron loading of RAW264.7 significantly increased with the concentration of the nanoparticles and the treatment time. However, both human liver cells (HepG2 and HL-7720) were labeled with the lowest iron loading. The measurement of cell viability revealed that the growth of all cells was not affected by the nanoparticles at a common in vivo application dose of iron nanoparticles (30 μg/mL), demonstrating that the nanoparticles have better biocompatability.