Ultrastructural observations of spermatogenesis following transplantation of rat testis cells into mouse seminiferous tubules.
Ultrastructural observations of spermatogenesis following transplantation of rat testis cells into mouse seminiferous tubules.
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DOI：
10.1002/j.1939-4640.1996.tb01845.x
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发表时间：
1996-11
影响因子：
--
通讯作者：
L. Russell;R. Brinster
中科院分区：
文献类型：
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作者：
L. Russell;R. Brinster
白消安处理的免疫缺陷小鼠接受大鼠睾丸细胞的生精小管注射的睾丸进行了检查，光镜和电镜。通过已知表征该种属生精细胞的标准验证了大鼠精子发生活跃。此外，由于白消安处理后精子发生重新启动，小鼠的精子发生被确定为发生在一些曲细精管中。小鼠曲细精管中的大鼠精子发生显示出已知表征大鼠精子发生阶段的细胞的普遍公认的关联。与大鼠精子发生有关的支持细胞经超微结构鉴定为小鼠起源。因此，大鼠精子发生的周期长度比小鼠精子发生的周期长度长50%，可以在小鼠支持细胞中进行，而支持细胞据推测与小鼠生殖细胞发育相一致，发挥更短的周期影响。需要研究以确定大鼠精子发生的时间是否由生殖细胞或支持细胞控制。这些观察结果被认为是初步的，因为没有进行体细胞-生殖细胞关系的彻底研究。结论：小鼠睾丸支持细胞在小鼠睾丸提供的环境中可以产生小鼠和大鼠的配子。
The testes of busulfan-treated immunodeficient mice receiving seminiferous tubule injections of testis cells from rats were examined by light and electron microscopy. The presence of active rat spermatogenesis was verified by criteria that are known to characterize spermatogenic cells of this species. In addition, spermatogenesis from the mouse was identified as taking place in some seminiferous tubules as the result of reinitiation of spermatogenesis after busulfan treatment. Rat spermatogenesis in mouse seminiferous tubules showed the generally recognized associations of cells known to characterize stages of spermatogenesis of the rat. The Sertoli cells associated with rat spermatogenesis were identified ultrastructurally as being of mouse origin. Thus, rat spermatogenesis, which has a cycle length that is 50% longer than mouse spermatogenesis, can proceed among mouse Sertoli cells, which supposedly exert much shorter cyclic influences in concert with mouse germ cell development. Studies are needed to determine if the timing of rat spermatogenesis is controlled by the germ cells or the Sertoli cells. These observations are considered preliminary since a thorough study of somatic-germ cell relationships was not undertaken. It is concluded that a mouse Sertoli cell in the environment provided by the mouse testis can produce both mouse and rat gametes.