Colonization and penetration of denture soft lining materials by Candida albicans
Colonization and penetration of denture soft lining materials by Candida albicans
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DOI：
10.1016/s0109-5641(03)00088-5
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发表时间：
2004-02-01
期刊：
影响因子：
5
通讯作者：
McCord, JF
中科院分区：
文献类型：
--
作者：
Bulad, K;Taylor, RL;McCord, JF
目标.白色念珠菌在义齿软衬材料上的定植可导致临床问题和材料的劣化。本研究旨在通过比较C.白色念珠菌的六种义齿衬里材料，并监测任何长期渗透材料的酵母。将牙科内衬材料（Molloplast B、Flexor、Permaflex、Luci-soft、Eversoft和Ufi Get hard C）对载玻片或牙科结石进行处理。将标准化（2.8 × 10（6）cfu/ml）洗涤细胞悬液与试验材料孵育1小时（37 ℃）后，监测酵母对表面的粘附。用吖啶橙子染色的附着细胞在显微镜下计数。通过使用吖啶橙子染色的切片，观察培养6周后酵母渗透到粘合到丙烯酸树脂上的材料中（每周更换培养基）。菌丝和酵母渗透估计（定性和定量，分别）的三个层次的内衬（地下，中央部分和相邻的内衬丙烯酸交界处）。与制霉菌素对照相比，没有一种材料产生抑制区。在任何光滑表面上的细胞数量没有显著差异（p > 0.5）。显著地，（p < 0.001）在粗糙表面上观察到更高数量的细胞。当监测渗透时，观察到菌丝和酵母形式。在Ufi Get hard C中的渗透最大（未观察到菌丝），但在丙烯酸-内衬连接处没有，在Eversoft中的渗透最小。不同的义齿衬里材料在对酵母菌渗透的敏感性方面表现出不同的特性，尽管初始附着力相当。更平滑的表面保留更少的单元。为给定功能选择合适的材料及其制造可能会影响性能。(C)2003年由Elsevier Ltd代表牙科材料学会出版。All rights reserved.
Objectives. Colonization of denture soft lining materials by Candida albicans can result in clinical problems, and deterioration of the material. This study aimed to monitor this interaction by comparing the short-term adhesion of C. albicans to six denture lining materials and to monitor any longer term penetration of material by the yeast.Methods. Denture lining materials (Molloplast B, Flexor, Permaflex, Luci-soft, Eversoft and Ufi Get hard C) were processed against glass slides or dental stone. Adhesion of yeast to surfaces was monitored after one hour incubation (37degreesC) of standardized (2.8 x 10(6) cfu/ml) washed cell suspension with test materials. Attached cells stained with acridine orange were counted microscopically. Penetration of yeast into materials bonded onto acrylic after six weeks incubation (culture medium was replaced weekly) was observed through sections stained using acridine orange. Hyphal and yeast penetration was estimated (qualitatively and quantitatively, respectively) for three levels of the liner (subsurface, central section and adjacent to lining-acrylic junction).Results. None of the materials produced a zone of inhibition when compared with the nystatin control. There was no significant difference (p > 0.5) in cell numbers on any of the smooth surfaces. Significantly, (p < 0.001) higher numbers of cells were observed on roughened surfaces. Both hyphal and yeast forms were observed when penetration was monitored. Penetration was greatest into Ufi Get hard C (no hyphae observed), but not at the acrylic-liner junction and least into Eversoft.Significance. Different denture lining materials exhibit different properties in terms of susceptibility to yeast penetration, although the initial attachment is comparable. Smoother surfaces retain fewer cells. The selection of appropriate materials for a given function, and their fabrication may affect performance. (C) 2003 Published by Elsevier Ltd on behalf of Academy of Dental Materials. All rights reserved.