Zygotic caudal regulation by hunchback and its role in abdominal segment formation of the Drosophila embryo.
Zygotic caudal regulation by hunchback and its role in abdominal segment formation of the Drosophila embryo.
复制标题
驼背对合子尾部的调节及其在果蝇胚胎腹节形成中的作用。
作者：
C. Schulz;Diethard Tautz
cad（caudal）是果蝇中的母系和合子表达基因，其中两个表达阶段可以在功能上相互替代。合子的表达形成了一个腹部和后部结构域，其中只有后部结构域迄今已被研究，就其调节和功能。我们在这里表明，腹部CAD域的驼背（HB）梯度通过抑制在高浓度和激活在低浓度的HB蛋白的调节。为了研究腹部cad结构域在缺乏冗余相互作用的情况下的功能，我们利用了一个胚胎缺乏正常bicoid（bcd）和hb表达的实验系统。然后将人工hb梯度引入到这样的胚胎中，这导致在更前部区域诱导异位合子cad结构域。利用这个系统，我们发现cad结构域通过激活腹隙基因knirps（kni）和giant（gt）的表达而发挥作用。我们的结论是cad是迄今为止缺失的腹部分割基因的区域特异性激活因子。
caudal (cad) is a maternally and zygotically expressed gene in Drosophila whereby the two phases of expression can functionally replace each other. The zygotic expression forms an abdominal and a posterior domain, whereby only the posterior domain has so far been studied with respect to its regulation and function. We show here that the abdominal cad domain is regulated by the hunchback (hb) gradient through repression at high concentrations and activation at low concentrations of HB protein. To study the function of the abdominal cad domain in the absence of redundant interactions, we have utilized an experimental system in which the embryo lacks the normal bicoid (bcd) and hb expression. An artificial hb gradient is then introduced into such embryos, which results in an induction of an ectopic zygotic cad domain in the more anterior region. Employing this system, we show that the cad domain functions by activating the expression of the abdominal gap genes knirps (kni) and giant (gt). We conclude that cad is the so far missing region-specific activator of abdominal segmentation genes.
果蝇间隙基因巨人与母本和合子模式形成基因的相互作用。
DOI：
10.1242/dev.111.2.367
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Eldon,ED;Pirrotta,V
通讯作者：
Pirrotta,V
间隙基因giant和Krüppel之间的相互抑制相互作用定义了果蝇胚胎的中体区域。
DOI：
10.1242/dev.111.2.611
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Kraut,R;Levine,M
通讯作者：
Levine,M