Repair of nasal defects using collagen gels containing insulin-like growth factor 1.
Repair of nasal defects using collagen gels containing insulin-like growth factor 1.
复制标题
使用含有胰岛素样生长因子 1 的胶原凝胶修复鼻部缺陷。
DOI：
10.1097/00005537-199811000-00012
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Lindsey,WH
中科院分区：
文献类型：
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作者：
Toung,JS;Griffin,A;Ogle,RC;Lindsey,WH
目的：面骨缺损是耳鼻咽喉头颈外科医生常见且具有挑战性的问题。目前的修复方法包括合成移植物、身体材料和自体组织，具有异物反应、感染性物质传播和二次手术部位的发病率等缺点。为了开发一种理想的骨重建技术，先前已经在Spraogue-Dawley大鼠身上开发出了临界大小的面部缺陷。该模型在6个月内的表面积愈合率不到10%。应用I型胶原凝胶和胰岛素样生长因子-1(IGF-1)，尝试了一种新的骨重建方法。研究设计：使用啮齿动物模型进行随机对照试验。方法：12只成年雄性SD大鼠接受外科手术，用切割毛刺切除鼻骨来制造临界大小的鼻骨缺损。6只动物用300μg的I型胶原凝胶修复。6只动物用300μg的I型胶原凝胶加3.0μg的胰岛素样生长因子-1修复。30天后，动物进行尸检。进行精确的平面测量、放射密度分析和组织切片。结果：所有动物都完全覆盖了这种缺损区，只有一层薄薄的骨层。放射密度分析表明，与单纯胶原组相比，胶原组和IGF-1组的骨密度有统计学意义的增加(P<；0.037)。此外，组织学评估显示IGF-1组的骨密度和厚度增加。结论：I型胶原凝胶与IGF-1增强可显著增加啮齿动物模型中鼻部临界大小缺损的愈合。喉镜，108：1654-1658,1998
Objective:Facial osseous defects are a common and challenging problem for the otolaryngologist—head and neck surgeon. Current methods of repair including synthetic grafts, cadaveric material, and autologous tissue have drawbacks of foreign body reactions, infectious agent transmission, and the morbidity of a second surgical site. In the effort to develop an ideal technique for osseous reconstruction, a critical‐size facial defect has previously been developed in the Sprague‐Dawley rat. This model exhibits less than 10% healing by surface area over 6 months. A novel approach to osseous reconstruction is attempted using this model with type I collagen gel augmented with insulin‐like growth factor 1 (IGF‐1).Study Design:Randomized controlled trial using a rodent model.Methods:Twelve adult male Sprague‐Dawley rats underwent a surgical procedure to produce a critical‐size nasal defect by removing the nasal bones with a cutting burr. Six animals were repaired with 300 μg of type I collagen gel. Six animals were repaired with 300 μg of type I collagen gel augmented with 3.0 μg of IGF‐1. Thirty days later, the animals were examined after necropsy. Precise planimetry, radiodensitometric analysis, and histologic sectioning were performed.Results:All animals had complete coverage of this defect with a thin layer of bone. Radiodensitometric analysis indicated that there was a statistically significant (P< .037) increase in bone density in the collagen plus IGF‐1 group compared with that of collagen only. In addition, histologic evaluation revealed increased bone density and thickness in the IGF‐1 group.Conclusion:Type I collagen gel augmented with IGF‐1 results in a significant increase in healing of a nasal critical‐size defect in a rodent model.Laryngoscope, 108:1654–1658, 1998