Immunophenotypic analysis of peripheral blood mononuclear cells undergoing in vitro apoptosis after isolation from human immunodeficiency virus-infected children
Immunophenotypic analysis of peripheral blood mononuclear cells undergoing in vitro apoptosis after isolation from human immunodeficiency virus-infected children
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DOI：
10.1182/blood.v92.11.4230.423k22_4230_4237
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发表时间：
1998-12-01
期刊：
影响因子：
20.3
通讯作者：
Pahwa, S
中科院分区：
文献类型：
--
作者：
McCloskey, TW;Bakshi, S;Pahwa, S
人类免疫缺陷病毒（HIV）感染者的淋巴细胞在体外经历加速凋亡，但受影响的细胞亚群尚未明确定义。本研究通过流式细胞术检查了艾滋病毒感染儿童淋巴细胞表型与凋亡细胞死亡之间的关系，使用表面抗原标记与Tdt介导的Utp缺口末端标记（TUNEL）同时进行的组合来直接检查凋亡淋巴细胞的表型。与活细胞相比，凋亡淋巴细胞显示出表达CD 45 RO和HLA-DR的细胞的过度表达，而表达CD 28和CD 95的细胞的表达不足。表达CD 4、CD 8和CD 38的淋巴细胞在凋亡和活细胞群中的比例相等。当培养后的淋巴细胞凋亡百分比与新鲜血液中的淋巴细胞亚群独立检查时，凋亡与CD 4细胞的百分比呈负相关，但与特异性CD 4 T细胞亚群无关。虽然与总CD 8细胞的百分比不相关，但凋亡与表达CD 45 RO和CD 95的特异性CD 8 T细胞亚群正相关，与表达CD 45 RA的CD 8 T细胞负相关。这些结果提供了直接的证据表明，一群活化的淋巴细胞的记忆表型缺乏共刺激分子CD 28是特别容易发生凋亡。在新鲜细胞和凋亡细胞中与CD 95表达相关的发现暗示了儿童HIV疾病中细胞凋亡的Fas依赖性和Fas非依赖性途径。(C)1998年，美国血液学会。
Lymphocytes of human immunodeficiency virus (HIV)infected individuals undergo accelerated apoptosis in vitro, but the subsets of cells affected have not been clearly defined. This study examined the relationship between lymphocyte phenotype and apoptotic cell death in HIV-infected children by flow cytometry, Direct examination of the phenotype of apoptotic lymphocytes was accomplished using a combination of surface antigen labeling performed simultaneously with the Tdt mediated Utp nick end-labeling (TUNEL) assay. In comparison to live cells, apoptotic lymphocytes displayed an overrepresentation of CD45RO and HLA-DR expressing cells, while CD28 and CD95 expressing cells were underrepresented. Lymphocytes expressing CD4, CD8, and CD38 were equally represented in apoptotic and live populations. When percent lymphocyte apoptosis following culture was examined independently with lymphocyte subsets in fresh blood, apoptosis was negatively correlated with the percentage of CD4 cells, but not with specific CD4 T-cell subsets. Although not correlated with the percentage of total CD8 cells, apoptosis was positively correlated with specific CD8 T-cell subsets expressing CD45RO and CD95 and negatively correlated for CD8 T cells expressing CD45RA. These results provide direct evidence that a population of activated lymphocytes with the memory phenotype lacking the costimulatory molecule CD28 are especially prone to undergo apoptosis. The findings related to CD95 expression in fresh and apoptotic cells implicate Fas-dependent and Fas-independent pathways of apoptosis in HIV disease in children. (C) 1998 by The American Society of Hematology.