Interrelationships between gill chloride cell morphology and calcium uptake in freshwater teleosts
Interrelationships between gill chloride cell morphology and calcium uptake in freshwater teleosts
复制标题
淡水硬骨鱼鳃氯化物细胞形态与钙吸收之间的相互关系
DOI：
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发表时间：
1992
影响因子：
2.9
通讯作者：
J. Fenwick
中科院分区：
文献类型：
--
作者：
S. Perry;G. Goss;J. Fenwick
研究了淡水鱼鳃氯细胞（CCs）对跨鳃钙吸收（JinCa 2+）的影响。这是通过评估暴露于外部环境的CC的顶端表面积和JinCa 2+之间的种间关系。三种淡水硬骨鱼，虹鳟鱼（虹鳟），美国鳗鱼（长吻鳗鲡）和棕色牛头鲶鱼（Ictalurus nebulosus），使用。慢性（10天）治疗皮质醇在每个物种被用来作为一种工具，以引起的变化，在JinCa 2+和鳃CC形态，以评估种内CC表面积和JinCa 2+之间的关系。基于扫描电镜分析的定量形态测量结果表明，鲶鱼鳃丝上皮细胞上的暴露CC面积分数（CCFA）最低（12，744 ± 2248 μm2/mm 2），其次为美洲鳗（21，355 ± 981 μm2/mm 2）和虹鳟鱼（149，928 ± 26，545 μm2/mm 2）。除鲶鱼外，皮质醇慢性治疗引起CCFA显着增加，由于CC的增殖和/或个别CC的扩大（鳗鱼）。JinCa ~（2+）的比值反映了各物种的CC分数面积。相关性分析结果表明，鳟鱼和鳗鱼的CC分数面积与JinCa 2+之间存在显著相关性。由于没有皮质醇治疗的影响，有鲶鱼没有显着的相关性，因为在这个物种的CC分数面积的变化不足。CC分数面积与JinCa 2+在3种植物中呈显著正相关。这些结果表明，CC是参与淡水硬骨鱼的钙吸收和钙吸收率的种内和种间差异可以解释的鳃上皮细胞上暴露的CC的表面积的变化。
The involvement of the freshwater fish gill chloride cells (CCs) in trans-branchial calcium uptake (JinCa2+) was investigated. This was accomplished by assessing the interspecific relationships between the apical surface area of CCs exposed to the external environment and JinCa2+. Three species of freshwater teleosts, the rainbow trout (Oncorhynchus mykiss), the American eel (Anguilla rostrata) and the brown bullhead catfish (Ictalurus nebulosus), were used. Chronic (ten-day) treatment with cortisol in each species was used as a tool to evoke variations in both JinCa2+ and gill CC morphology in order to assess intraspecific relationships between CC surface area and JinCa2+. The results of quantitative morphometry, based on analysis of scanning electron micrographs, demonstrated that catfish possessed the lowest fractional area of exposed CC (CCFA) on the gill filament epithelium (12,744 ± 2248 μm2/mm2) and was followed, in increasing order, by American eel (21,355 ± 981 μm2/mm2) and rainbow trout (149,928 ± 26,545 μm2/mm2). With the exception of catfish, chronic treatment with cortisol caused significant increases in CCFA owing to proliferation of CCs and/or enlargement of individual CCs (eel only). The rates of JinCa2+ closely reflected the CC fractional area in each species. The results of correlation analysis revealed significant correlations between CC fractional area and JinCa2+ in trout and eel. Owing to the absence of an effect of cortisol treatment, there was no significant correlation in catfish because of insufficient variation in CC fractional area in this species. CC fractional area was significantly correlated with JinCa2+ among the three species examined. These results suggest that CC is involved in calcium uptake in freshwater teleosts and that both intra- and interspecific differences in the rates of calcium uptake can be accounted for by variability in the surface area of exposed CCs on the gill epithelia.
分离的鱼鳃细胞的代谢：上皮氯细胞的贡献。
DOI：
10.1242/jeb.144.1.507
发表时间：
1989
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Perry,SF;Walsh,PJ
通讯作者：
Walsh,PJ