Enhanced (S)-linalool production by fusion expression of farnesyl diphosphate synthase and linalool synthase in Saccharomyces cerevisiae
Enhanced (S)-linalool production by fusion expression of farnesyl diphosphate synthase and linalool synthase in Saccharomyces cerevisiae
复制标题
通过在酿酒酵母中融合表达法尼基二磷酸合酶和芳樟醇合酶来增强 (S)-芳樟醇生产
DOI：
10.1111/jam.13105
复制
发表时间：
2016-07-01
影响因子：
4
通讯作者：
Zhou, Jingwen
中科院分区：
文献类型：
--
作者：
Deng, Yu;Sun, Mingxue;Zhou, Jingwen
目的为了提高甲羟戊酸途径中香叶基二磷酸 (GPP) 的利用率，以增强酿酒酵母 (S)- 芳樟醇的生产。方法和结果A (S)-芳樟醇合酶 (LIS)：来自软枣猕猴桃的 AaLS1 与具有不同肽接头的 FPPS 共表达，以重定向从香叶基二磷酸 (GPP) 到 (S)-芳樟醇生产的通量在酿酒酵母中。具有最佳肽接头((GGGGS)(3))的菌株产生了10155297gl(-1)(S)-芳樟醇，与具有两个独立表达的LIS和FPPS的菌株相比增加了697%。在3升发酵罐中，(S)-芳樟醇滴度进一步提高至24064531gl(-1)。结论结果表明，融合蛋白催化代谢途径中的连续步骤，显着提高了以GPP为前体的(S)-芳樟醇产量。研究的意义和影响共表达AaLS1和FPPS的融合蛋白策略，与长肽接头组装使酿酒酵母产生迄今为止报道的最高 (S)-芳樟醇滴度。
AimsIn order to improve the availability of geranyl diphosphate (GPP) in the mevalonate pathway for enhancing (S)-linalool production in Saccharomyces cerevisiae.Methods and ResultsA (S)-linalool synthase (LIS): AaLS1 from Actinidia arguta was coexpressed with FPPS with different peptide linkers to redirect the flux from geranyl diphosphate (GPP) to (S)-linalool production in S.cerevisiae. The strain with the best peptide linker ((GGGGS)(3)), produced 10155297gl(-1) (S)-linalool, a 697% increase compared to those with two independent LIS and FPPS expressed. In a 3-l fermenter, the (S)-linalool titre was further improved to 24064531gl(-1).ConclusionsThe results demonstrate that the fusion proteins catalysing consecutive steps in a metabolic pathway significantly improved the (S)-linalool production with GPP as precursor.Significance and Impact of the StudyThe fusion protein strategy co-expressing AaLS1 and FPPS, assembled with a long peptide linker made S.cerevisiae produced the highest reported (S)-Linalool titre to date.