A new technique to investigate cell layers of the capsule wall using Frullania (hepaticae) as a case study

A new technique to investigate cell layers of the capsule wall using Frullania (hepaticae) as a case study
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DOI:
10.2307/3244363
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发表时间:
1999-06-01
期刊:
影响因子:
0.9
通讯作者:
Harris, PJ
Harris, PJ
中科院分区:
生物学4区
文献类型:
--
作者:
von Konrat, M;Braggins, JE;Harris, PJ

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据报道,一种新技术可用于检查肝脏囊壁不同层的解剖特征。该技术涉及用果胶酶制剂处理胶囊以分离各层,然后进行透明化和染色以增强细胞壁结构的细节。作为案例研究,讨论了一些新西兰 Frullania 物种的胶囊壁特征的潜在分类学价值。特别重要的特征包括内层和表皮层细胞壁增厚的形态。还讨论了胶囊壁层的细胞壁增厚中可能存在的化学成分。
A new technique is reported for examining anatomical features of the different layers of the capsule wall in hepatics. This technique involves treating the capsule with a pectinase preparation to separate the layers, followed by clearing and staining to enhance detail of the cell wall architecture. The potential taxonomic value of capsule wall characters for some New Zealand Frullania species is discussed as a case study. Characters of particular significance include the morphology of cell wall thickenings in both the internal and epidermal layers. The possible chemical components present in the cell wall thickenings of the capsule wall layers are also discussed.