Glial Cells Activation Potentially Contributes to the Upregulation of Stromal Cell-Derived Factor-1α After Optic Nerve Crush in Rats
Glial Cells Activation Potentially Contributes to the Upregulation of Stromal Cell-Derived Factor-1α After Optic Nerve Crush in Rats
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DOI：
10.1007/s11064-013-1106-0
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发表时间：
2013-07
影响因子：
4.4
通讯作者：
Xi-Tao Yang;Dong-chao Pan;Er-tao Chen;Yong-yan Bi;D. Feng
中科院分区：
文献类型：
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作者：
Xi-Tao Yang;Dong-chao Pan;Er-tao Chen;Yong-yan Bi;D. Feng
基质细胞衍生因子-1 α（SDF-1α）在创伤后起重要作用。然而，很少有人知道它的时间和空间的表达模式，或如何与胶质细胞相互作用后，视神经挤压损伤。我们描述了视神经挤压后视网膜和视神经中SDF-1α的时空表达模式，并证明SDF-1α定位于分布在视网膜和视神经中的胶质细胞。CXCR 4是SDF-1α的受体，沿着神经节细胞层（GCL）表达。分别采用实时荧光定量PCR和酶联免疫吸附法检测伤后1、2、3、5、7、10和14 d视网膜和视神经Sdf-1α mRNA和SDF-1α蛋白的相对表达水平，并采用实时荧光定量PCR检测Cxcr 4 mRNA的表达水平。采用免疫荧光和免疫组织化学方法检测损伤后SDF-1α和CXCR 4的定位。Sdf-1α和Cxcr 4 mRNA在视网膜和视神经中的表达分别在伤后第1天和第2天开始上调，在伤后5-7天达到高峰。Sdf-1α和Cxcr 4 mRNA在视神经挤压伤后至少14 d仍有表达。此外，SDF-1α阳性区在反应性胶质细胞中局部分布，提示可能存在自分泌刺激。CXCR 4主要表达于GCL中，与胶质细胞相邻。这些发现表明，视神经挤压后，视网膜和视神经中内源性SDF-1α和CXCR 4水平增加，其中活化的胶质细胞可能是SDF-1α蛋白增加的来源。
Stromal cell-derived factor-1α (SDF-1α) plays an important role after injury. However, little is known regarding its temporal and spatial expression patterns or how it interacts with glial cells after optic nerve crush injury. We characterized the temporal and spatial expression pattern of SDF-1α in the retina and optic nerve following optic nerve crush and demonstrated that SDF-1α is localized to the glial cells that are distributed in the retina and optic nerve. CXCR4, the receptor for SDF-1α, is expressed along the ganglion cell layer (GCL). The relative expression levels of Sdf-1α mRNA and SDF-1α protein in the retina and optic nerve 1, 2, 3, 5, 7, 10 and 14 days after injury were determined using real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay, respectively, and the Cxcr4 mRNA expression was determined using real-time PCR. Immunofluorescence and immunohistochemical approaches were used to detect the localization of SDF-1α and CXCR4 after injury. The upregulation of Sdf-1α and Cxcr4 mRNA was detected as early as day one after injury in the retina and day two in the optic nerve, the expression peaks 5–7 days after injury. The expression of Sdf-1α and Cxcr4 mRNA was maintained for at least 14 days after the optic nerve crush injury. Furthermore, SDF-1α-positive zones were distributed locally in the reactive glial cells, which suggested potential autocrine stimulation. CXCR4 was mainly expressed in the GCL, which was also adjacent to the the glial cells. These findings suggest that following optic nerve crush, the levels of endogenous SDF-1α and CXCR4 increase in the retina and optic nerve, where activated glial cells may act as a source of increased SDF-1α protein.