Changes in proteoglycan biosynthesis following leukocyte elastase treatment of bovine articular cartilage in culture.
Changes in proteoglycan biosynthesis following leukocyte elastase treatment of bovine articular cartilage in culture.
复制标题
白细胞弹性蛋白酶处理培养的牛关节软骨后蛋白多糖生物合成的变化。
作者：
J. Bartholomew;D. A. Lowther;C. Handley
用从兔多形核白细胞纯化的低分子量弹性蛋白酶处理培养物中的牛关节软骨，导致蛋白多糖从组织中降解和释放，同时长期抑制蛋白多糖生物合成。这些观察结果与在兔子诱发的实验性关节炎中观察到的结果一致。从经弹性蛋白酶处理的培养软骨中提取的蛋白多糖降解产物的大小与从关节炎软骨中提取的蛋白多糖降解产物的大小进行比较，显示出明显的相似性。结果强烈表明，白细胞弹性蛋白酶是炎症性关节疾病中蛋白聚糖降解和合成抑制的促成因素。
Treatment of bovine articular cartilage in culture with a low molecular weight elastase purified from rabbit polymorphonuclear leukocytes resulted in degradation and release of proteoglycans from the tissue, coupled with a prolonged inhibition of proteoglycan biosynthesis. These observations are consistent with those seen in experimental arthritis induced in rabbits. Comparison of the size of the proteoglycan degradation products extracted from elastase-treated cartilage in culture with that from arthritic cartilage showed marked similarities. The results strongly suggest that leukocyte elastase is a contributing factor in proteoglycan degradation and inhibition of synthesis in inflammatory joint disease.