Lactobacillus mucosae sp nov., a new species with in vitro mucus-binding activity isolated from pig intestine
Lactobacillus mucosae sp nov., a new species with in vitro mucus-binding activity isolated from pig intestine
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DOI：
10.1099/00207713-50-1-251
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发表时间：
2000-01-01
影响因子：
2.8
通讯作者：
Jonsson, H
中科院分区：
文献类型：
--
作者：
Roos, S;Karner, F;Jonsson, H
从猪小肠中分离鉴定出一种新的乳酸杆菌。为了分离携带推定的定殖因子基因（mub，用于粘液结合）的罗伊氏乳杆菌菌株，使用mub衍生的基因探针筛选猪肠材料。初步鉴定结果表明，这些菌株为革兰氏阳性、过氧化氢酶阴性、不产芽孢、不动杆菌。生长发生在45 ° C，但不是在15 ° C和DNA G+C含量为46摩尔%。细胞壁分析、DNA-DNA杂交和165 rRNA序列分析表明，新分离株代表了以前未描述的与L. reuteri。发酵乳杆菌和桥脑乳杆菌。建议将该菌种命名为粘膜乳杆菌，模式菌株为S32（T）。
A new Lactobacillus species from pig small intestine has been identified. In an attempt to isolate Lactobacillus reuteri strains carrying the putative colonization-factor gene (mub, for mucus binding) a mub-derived gene probe was used to screen pig intestinal material. A number of isolates were obtained and primary characterization showed that they were Cram-positive, catalase-negative, non-spore-forming, non-motile rods. Growth occurred at 45 degrees C but not at 15 degrees C and the DNA G+C content was 46 mol%. Cell wall analysis together with DNA-DNA hybridization and analysis of the 165 rRNA sequence revealed that the new isolates represent a previously undescribed Lactobacillus species closely related to L. reuteri. Lactobacillus fermentum and Lactobacillus pontis. The name Lactobacillus mucosae is proposed for this species and the type strain is S32(T).