Fluorescent in situ hybridization for monosomy 3 via 30-gauge fine-needle aspiration biopsy of choroidal melanoma in vivo
Fluorescent in situ hybridization for monosomy 3 via 30-gauge fine-needle aspiration biopsy of choroidal melanoma in vivo
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DOI：
10.1016/j.ophtha.2006.06.040
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发表时间：
2007-01-01
期刊：
影响因子：
13.7
通讯作者：
Straatsma, Bradley R.
中科院分区：
文献类型：
--
作者：
Young, Tara A.;Rao, Nagesh P.;Straatsma, Bradley R.
目的：报道术中经巩膜细针穿刺活检获得用于脉络膜黑色素瘤患者单体3分析的细胞的可行性。设计：连续介入病例系列。参与者：18例脉络膜黑色素瘤患者（18只眼睛），在碘125斑块放置时使用30号针进行细针穿刺活检。干预：从活检标本中获得3号单体的细胞学和细胞遗传学分析。主要观察指标：细胞学、3号单体细胞遗传学分析、活体脉络膜黑色素瘤经巩膜细针穿刺活检的并发症及可行性。结果：细针穿刺活检在18例中诊断出14例脉络膜黑色素瘤，在9例中产生活细胞培养用于荧光原位杂交（FISH）分析。3号单体荧光原位杂交4例阳性。一名患者有轻微的玻璃体出血。高度在2到3mm之间的肿瘤和那些在培养中产生的细胞没有附着的肿瘤最有可能没有足够的生长来进行FISH分析。结论：经巩膜细针穿刺活检和3号单体的FISH可能为接受斑块放疗的脉络膜黑色素瘤患者提供重要的预后信息。
Objective: To report the feasibility of intraoperative transscleral fine-needle aspiration biopsy at plaque surgery to obtain cells for monosomy 3 analysis in patients with choroidal melanoma.Design: Consecutive interventional case series.Participants: Eighteen patients (18 eyes) with choroidal melanoma who had fine-needle aspiration biopsy performed with a 30-gauge needle at time of iodine 125 plaque placement.Intervention: Cytology and cytogenetic analysis for monosomy 3 were obtained from biopsy specimens.Main Outcome Measures: Cytology, cytogenetic analysis for monosomy 3, and complications and feasibility of transscleral fine-needle aspiration biopsy of choroidal melanoma in vivo.Results: Fine-needle aspiration biopsy was diagnostic of choroidal melanoma in 14 of 18 cases and resulted in viable cell cultures for fluorescent in situ hybridization (FISH) analysis in 9 cases. Fluorescent in situ hybridization for monosomy 3 was positive in 4 of the 9 cases. One patient had a mild vitreous hemorrhage. Tumors between 2 and 3 mm in height and those that yielded cells that did not attach in culture were most likely to have insufficient growth for FISH analysis.Conclusions: Transscleral fine-needle aspiration biopsy and FISH for monosomy 3 may provide important prognostic information on patients who undergo plaque radiotherapy for choroidal melanoma.