Target-dependent specification of the neurotransmitter phenotype:: cholinergic differentiation of sympathetic neurons is mediated in vivo by gp130 signaling
Target-dependent specification of the neurotransmitter phenotype:: cholinergic differentiation of sympathetic neurons is mediated in vivo by gp130 signaling
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DOI：
10.1242/dev.02189
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发表时间：
2006-01-01
期刊：
影响因子：
4.6
通讯作者：
Rohrer, H
中科院分区：
文献类型：
--
作者：
Stanke, M;Duong, CV;Rohrer, H
交感神经元是通过一系列分化步骤产生的，这些分化步骤最初导致去甲肾上腺素能神经元支配不同的外周靶组织。特定目标，例如啮齿动物脚垫中的汗腺，会引起从去甲肾上腺素能递质表型向胆碱能递质表型的变化。在这里，我们证明汗腺中存在通过 gp130 受体发挥作用的细胞因子。选择性消除交感神经元中的 gp130 受体可防止获得胆碱能和肽能特征（VAChT、ChT1、VIP），而不影响汗腺神经支配的其他特性。 gp130 缺陷小鼠的星状神经节中绝大多数出生后产生的胆碱能神经元不存在。这些结果证明了 gp130 信号传导在胆碱能神经递质表型的靶标依赖性规范中的重要作用。
Sympathetic neurons are generated through a succession of differentiation steps that initially lead to noradrenergic neurons innervating different peripheral target tissues. Specific targets, like sweat glands in rodent footpads, induce a change from noradrenergic to cholinergic transmitter phenotype. Here, we show that cytokines acting through the gp130 receptor are present in sweat glands. Selective elimination of the gp130 receptor in sympathetic neurons prevents the acquisition of cholinergic and peptidergic features (VAChT, ChT1, VIP) without affecting other properties of sweat gland innervation. The vast majority of cholinergic neurons in the stellate ganglion, generated postnatally, are absent in gp130-deficient mice. These results demonstrate an essential role of gp130-signaling in the target-dependent specification of the cholinergic neurotransmitter phenotype.