Fine scale mapping places DLG1, the gene encoding hDlg, telomeric to the OPA1 candidate region.
Fine scale mapping places DLG1, the gene encoding hDlg, telomeric to the OPA1 candidate region.
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精细尺度映射将编码 hDlg 的基因 DLG1、端粒定位到 OPA1 候选区域。
DOI：
10.1007/s003359900578
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Chishti,AH
中科院分区：
文献类型：
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作者：
Alexander,C;Stathakis,DG;Lin,L;Rahman,S;Bryant,PJ;Auburger,G;Chishti,AH
图谱位置：近端–D13Mit1–1.06 cM±1.06 SE–Mtr、D13Bir4、D13Bir6–1.06±1.06–D13Abb1e–2.13±1.49–D13Bir7–远端 作图方法：通过对来自种间回交组的 96 只动物进行 RFLP 分析来定位 Mtr ((C57BL/6JEi× SPRET/Ei) F1× SPRET/Ei) 由杰克逊实验室提供，巴港，缅因州。(BSS panel)[1]。数据库存放信息：数据可从小鼠基因组数据库获得，登录号 MGD-JNUM-39061。分子试剂：通过小鼠肝脏 RNA 的逆转录/PCR 获得 1095 bp 的小鼠 cDNA，其中包含基于低等生物甲硫氨酸合酶序列内同源区域的简并寡核苷酸。正如 Leclerc 等人[2]所述，这两种引物（D1730 和 D1733）成功扩增了人和小鼠 cDNA。对两个物种的 PCR 产物进行亚克隆和测序；他们表现出 89% 的同一性。小鼠 cDNA 通过随机引发进行标记，并与 EcoRI 消化的小鼠基因组 DNA 的 Southern 印迹杂交。等位基因检测：通过EcoRI多态性的RFLP分析进行等位基因检测。 C57BL/6J菌株具有约13kb的等位基因，而Mus spretus菌株具有约9kb和4kb的等位基因。在两种菌株中均观察到约 0.5 kb 的恒定条带。
Map position: proximal–D13Mit1–1.06 cM±1.06 SE–Mtr, D13Bir4, D13Bir6–1.06±1.06–D13Abb1e–2.13±1.49–D13Bir7–distal Method of mapping: Mtr was localized by RFLP analysis of 96 animals from an interspecific backcross panel ((C57BL/6JEi× SPRET/Ei) F1× SPRET/Ei) provided by The Jackson Laboratory, Bar Harbor, Me.(BSS panel)[1].Database deposit information: The data are available from the Mouse Genome Database, accession number MGD-JNUM-39061. Molecular reagents: A 1095-bp mouse cDNA was obtained by reverse transcription/PCR of mouse liver RNA, with degenerate oligonucleotides based on regions of homology within the methionine synthase sequences of lower organisms. The two primers (D1730 and D1733), as described by Leclerc et al.[2], were successful in amplifying both human and mouse cDNAs. The PCR products from both species were subcloned and sequenced; they showed 89% identity. The mouse cDNA was labeled by random priming and hybridized to Southern blots of EcoRI-digested mouse genomic DNA. Allele detection: Allele detection was performed by RFLP analysis of an EcoRI polymorphism. The C57BL/6J strain has alleles of approximately 13 kb, while the Mus spretus strain has alleles of approximately 9 kb and 4 kb. A constant band of approximately 0.5 kb was seen in both strains.