H2O2 mobilizes Ca2+ from agonist- and thapsigargin-sensitive and insensitive intracellular stores and stimulates glutamate secretion in rat hippocampal astrocytes
H2O2 mobilizes Ca2+ from agonist- and thapsigargin-sensitive and insensitive intracellular stores and stimulates glutamate secretion in rat hippocampal astrocytes
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DOI：
10.1007/s11064-006-9078-y
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发表时间：
2006-06-01
影响因子：
4.4
通讯作者：
Salido, Gines M.
中科院分区：
文献类型：
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作者：
Gonzalez, Antonio;Granados, Maria P.;Salido, Gines M.
本研究旨在观察过氧化氢（H_2O_2）对大鼠海马星形胶质细胞胞浆游离钙浓度（[Ca ~（2+）]（c））及谷氨酸（Glu）分泌的影响。我们的研究结果表明，100 μ M H2 O2诱导增加[Ca 2 +]（c），保持在一个升高的水平，而氧化剂是存在于灌注介质中，由于其从细胞内存储的释放，因为它被观察到在没有细胞外a，随后由一个显着增加谷氨酸分泌。钙动员响应氧化剂只能减少毒胡萝卜素加FCCP，表明钙动员池H2 O2包括内质网和线粒体。我们得出结论，海马星形胶质细胞中的ROS可能有助于静息[Ca 2 +]（c）的升高，这反过来又可能导致兴奋性神经递质谷氨酸的持续分泌，这被认为是可能导致海马神经毒性的情况。
The effect of hydrogen peroxide (H2O2) on cytosolic free calcium concentration ([Ca2+](c)) as well as its effect on glutamate secretion in rat hippocampal astrocytes have been the aim of the present research. Our results show that 100 mu M H2O2 induces an increase in [Ca2+](c) that remains at an elevated level while the oxidant is present in the perfusion medium, due to its release from intracellular stores as it was observed in the absence of extracellular a, followed by a significant increase in glutainate secretion. Ca2+-mobilization in response to the oxidant could only be reduced by thapsigargin plus FCCP, indicating that the Ca2+-mobilizable pool by H2O2 includes both endoplasmic reticulum and mitochondria. We conclude that ROS in hippocampal astrocytes might contribute to an elevation of resting [Ca2+](c) which, in turn, could lead to a maintained secretion of the excitatory neurotransmitter glutamate, which has been considered a situation potentially leading to neurotoxicity in the hippocampus.