Quinacrine Enhances Vesicular Stomatitis Virus Inactivation and Diminishes Hemolysis of Dimethylmethylene Blue–phototreated Red Cells¶
Quinacrine Enhances Vesicular Stomatitis Virus Inactivation and Diminishes Hemolysis of Dimethylmethylene Blue–phototreated Red Cells¶
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奎纳克林增强水疱性口炎病毒灭活并减少二甲基亚甲基蓝光处理红细胞的溶血¶
DOI：
10.1562/0031-8655(2002)0760514qevsvi2.0.co2
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
D. Thompson‐Montgomery
中科院分区：
文献类型：
--
作者：
S. Wagner;A. Skripchenko;D. Thompson‐Montgomery
摘要几种用于红细胞（RBC）悬浮液中病毒灭活的光动力学方法在1-6°C长期储存期间导致不必要的溶血。为了探索溶血可能由膜结合染料介导的可能性，在病毒光灭活和光溶血研究中，使用结构与光敏剂相似但光吸收性质不同的分子作为RBC膜结合的抑制剂。在用3.6 μM二甲基亚甲基蓝（DMMB）和219 mJ/cm 2红光处理之前，向氧合RBC中加入500 μM奎纳克林，导致增敏剂的细胞外浓度增加，细胞外病毒灭活动力学增加，并在1-6°C储存期间减少溶血，而不改变奎纳克林吸收特性。这些结果共同表明，尽管DMMB对病毒核酸具有公认的亲和力，但它也与RBC膜结合，并且结合的染料部分地负责光诱导的溶血。
Abstract Several photodynamic methods for virus inactivation in red blood cell (RBC) suspensions have resulted in unwanted hemolysis during extended 1–6°C storage. To explore the possibility that hemolysis may be mediated by a membrane-bound dye, a molecule similar in structure to yet different in light absorption properties from the photosensitizer was used as an inhibitor for RBC membrane binding in virus photoinactivation and photohemolysis studies. The addition of 500 μM quinacrine to oxygenated RBC before treatment with 3.6 μM dimethylmethylene blue (DMMB) and 219 mJ/cm2 red light resulted in an increased extracellular concentration of the sensitizer, increased extracelluar viral inactivation kinetics, and decreased hemolysis during 1–6°C storage without alteration of quinacrine absorption properties. These results collectively suggest that despite its recognized affinity for viral nucleic acid, DMMB also binds to RBC membranes and that the bound dye is, in part, responsible for photoinduced hemolysis.
部花青 540 敏化光照射作为灭活血液制品中包膜病毒的方法的评估。
DOI：
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发表时间：
1990
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
O'Brien,JM;Montgomery,RR;Burns,WH;Gaffney,DK;Sieber,F
通讯作者：
Sieber,F
使用铝酞菁 (AIPc) 磺酸盐灭活红细胞和血小板浓缩液中的病毒。
DOI：
--
发表时间：
1992
期刊：
Blood cells
影响因子：
--
作者：
Horowitz,B;Rywkin,S;Margolis-Nunno,H;Williams,B;Geacintov,N;Prince,AM;Pascual,D;Ragno,G;Valeri,CR;Huima-Byron,T
通讯作者：
Huima-Byron,T
鼠伤寒沙门氏菌 9-氨基吖啶衍生物的移码诱变。
DOI：
10.1016/0165-1218(81)90045-8
发表时间：
1981
期刊：
Mutation research
影响因子：
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作者：
Young,PR;Ma,RI;Marfey,P;Kallenbach,NR
通讯作者：
Kallenbach,NR