A tyrosine sulfated human glycoprotein with an unusual cell distribution.
A tyrosine sulfated human glycoprotein with an unusual cell distribution.
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一种酪氨酸硫酸化人糖蛋白，具有不寻常的细胞分布。
DOI：
10.1016/0161-5890(92)90151-m
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发表时间：
1992
影响因子：
3.6
通讯作者：
Kagnoff,MF
中科院分区：
文献类型：
--
作者：
Omary,MB;deGrandpre,L;Varki,NM;Kagnoff,MF
我们描述了一种被单克隆抗体M2B3识别的人整体细胞表面糖蛋白（Mr142 kD）。这种糖蛋白在静止的外周血淋巴细胞中不存在，但在有丝分裂原激活时显著表达。M2B3糖蛋白存在于表皮和食管组织基底层的上皮细胞以及几种新肿瘤中。此外，它存在于整个胃肠道的平滑肌组织中，但在其他组织来源的平滑肌，骨骼肌和心肌中不存在。M2B3糖蛋白与转化相关的糖蛋白Q14相似，但不完全相同。此外，M2B3和Q14种具有抗原性亲缘关系。尽管如此，基于细胞分布和部分肽定位的明显差异，M2B3和Q14是不同的糖蛋白。本文描述的M2B3抗原在酪氨酸上被硫酸化，并且代表迄今为止所描述的在酪氨酸残基上被硫酸化的少数细胞表面蛋白之一。我们的研究表明，M2B3糖蛋白的功能可能与细胞粘附的细胞增殖有关。
We describe a human integral cell surface glycoprotein (Mr142 kD), recognized by monoclonal antibody M2B3. This glycoprotein is absent from resting peripheral blood lymphocytes, but becomes expressed in significant levels with mitogen activation. The M2B3 glycoprotein is present on epithelial cells in the basal layer of epidermal and esophageal tissue as well as in several fresh tumors examined. In addition, it is present on smooth muscle tissue throughout the gastrointestinal tract, but is absent from smooth muscle from several other tissue sources, skeletal muscle and cardiac muscle. The M2B3 glycoprotein is similar, but not identical, in apparent Mrto a transformation-associated glycoprotein, Q14. Further, the M2B3 and Q14 species are related antigenically. Nonetheless, M2B3 and Q14 are distinct glycoproteins based on clear differences in cell distribution and in partial peptide mapping. The M2B3 antigen described herein is sulfated on tyrosine, and represents one of the few cell surface proteins described to date that is sulfated on tyrosine residues. Our studies suggest the function of the M2B3 glycoprotein is likely to be associated with cell proliferation of cell adhesion.