X-ray radiation at low doses stimulates differentiation and mineralization of mouse calvarial osteoblasts
X-ray radiation at low doses stimulates differentiation and mineralization of mouse calvarial osteoblasts
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DOI：
10.5483/bmbrep.2012.45.10.101
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发表时间：
2012-10-31
期刊：
影响因子：
3.8
通讯作者：
Lee, Jeong-Chae
中科院分区：
文献类型：
--
作者：
Park, Soon-Sun;Kim, Kyoung-A;Lee, Jeong-Chae
放疗被认为对骨组织造成有害影响，最终增加骨质流失和骨折风险。然而，照射本身对成骨细胞的真实影响存在很大争议，照射影响成骨细胞分化和矿化的机制也不完全清楚。我们探讨了x射线辐射如何影响小鼠颅骨成骨细胞的分化和骨特异性基因表达。2 Gy辐照不仅能促进细胞分化和矿化，还能上调分化早期碱性磷酸酶、I型胶原、骨桥蛋白和骨钙素的表达。然而，低剂量（bbb20 Gy）的辐照并没有刺激成骨细胞分化，而是抑制了细胞的DNA合成，但没有毒性作用。其他实验表明，转化生长因子- β 1和转录因子- 2作为骨特异性标记物的上游调节因子，在辐照刺激的骨分化中发挥重要作用。[BMB报告2012；45 (10): 571 - 576)
Radiotherapy is considered to cause detrimental effects on bone tissue eventually increasing bone loss and fracture risk. However, there is a great controversy on the real effects of irradiation itself on osteoblasts, and the mechanisms by which irradiation affect osteoblast differentiation and mineralization are not completely understood. We explored how X-ray radiation influences differentiation aid bone-specific gene expression in mouse calvarial osteoblasts. Irradiation at 2 Gy not only increased differentiation and mineralization of the cells, but also upregulated the expression of alkaline phosphatase, type I collagen, osteopontin, and osteocalcin at early stages of differentiation. However, irradiation at hither doses (>2 Gy) did not stimulate osteoblast differentiation, rather it suppressed DNA synthesis by the cells without a toxic effect. Additional experiments suggested that transforming growth factor-beta 1 and runt-transcription factor 2 play important roles in irradiation- stimulated bone differentiation by acting as upstream regulators of bone-specific markers. [BMB Reports 2012; 45(10): 571-576]