Evidence for the early onset of gingival inflammation following short-term plaque accumulation
Evidence for the early onset of gingival inflammation following short-term plaque accumulation
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DOI：
10.1034/j.1600-051x.2002.291206.x
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发表时间：
2002-12-01
影响因子：
6.7
通讯作者：
Lingström, P
中科院分区：
文献类型：
--
作者：
Zhang, J;Kashket, S;Lingström, P
目的：开展一项研究，检测菌斑积累早期阶段龈沟液 (GCF) 中的细胞因子标记物。方法：由 5 名口腔卫生良好的受试者组成，1 至 3 天不刷牙，然后将纸条放入上颌前磨牙和第一磨牙的牙龈边缘 30 秒，采集 GCF 样本。 GCF流速用Periotron 仪器(Oraflow，Inc.，Plainview，New York)测定，并且嗜中性粒细胞(多形核白细胞)测定为髓过氧化物酶活性。从试纸条上洗脱白细胞介素-1b (IL-1b) 和 IL-8，并使用酶联免疫吸附测定 (ELISA) 系统进行测定。结果：3 天不刷牙后，菌斑指数升至 2.7 +/- 0.2（平均值 +/- SE），GCF 流速升至基线的 146.8%。 PMN 和 IL-8 浓度有所下降，但由于 GCF 流量增加而进行稀释校正后，与基线没有统计学差异。 IL-1b 在 1 天后略有升高，并在斑块积累 3 天后增加至 223.8 +/- 54.3%（从 6.8 +/- 1.7 至 13.8 +/- 3.6 pg/30 s；p = 0.04）。恢复刷牙导致 IL-1b 恢复至基线（刷牙 2 天后为 109.1%）。当受试者在 3 天不刷牙期间用 0.12% 洗必泰冲洗时，菌斑指数、GCF 流速和 IL-1b 释放率并未出现增加。结论：结果表明，在出现任何临床炎症症状之前，菌斑积聚 3 天后，GCF 中的 IL-1b 释放率增加。
Aim: A study was undertaken to examine cytokine markers in gingival crevicular fluid (GCF) during the early stages of plaque accumulation.Methods: A panel of five subjects with good oral hygiene went without brushing for 1 or 3 days, after which GCF samples were taken by placing paper strips into the gingival margins of the maxillary premolars and first molar for 30 s. GCF flow rates were determined with a Periotron(R) instrument (Oraflow, Inc., Plainview, New York), and neutrophils (polymorphonuclear leukocytes) were determined as myeloperoxidase activity. Interleukin-1b (IL-1b) and IL-8 were eluted from the paper strips and assayed with enzyme-linked immunosorbent assay (ELISA) systems.Results: The plaque index rose to 2.7 +/- 0.2 (mean +/- SE) after 3 days without brushing, and the GCF flow rate increased to 146.8% of baseline. PMN and IL-8 concentrations fell but, when corrected for dilution as a result of increased GCF flow, were not statistically different from baseline. IL-1b was slightly elevated after 1 day, and increased to 223.8 +/- 54.3% (from 6.8 +/- 1.7 to 13.8 +/- 3.6 pg/30 s; p = 0.04) after 3 days of plaque accumulation. Resumption of tooth brushing led to a return of IL-1b to baseline (109.1% after 2 days of brushing). When subjects rinsed with 0.12% chlorhexidine during the 3-day no-brushing period, the increases in plaque index, GCF flow rates and IL-1b release rates did not occur.Conclusions: The results indicate that IL-1b release rates increase in the GCF after 3 days of plaque accumulation, before any clinical signs of inflammation appear.