Lack of Maf1 enhances pyruvate kinase activity and fermentative metabolism while influencing lipid homeostasis in Saccharomyces cerevisiae
Lack of Maf1 enhances pyruvate kinase activity and fermentative metabolism while influencing lipid homeostasis in Saccharomyces cerevisiae
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DOI：
10.1002/1873-3468.12033
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发表时间：
2016-01-01
期刊：
影响因子：
3.5
通讯作者：
Chreptowicz, Karolina
中科院分区：
文献类型：
--
作者：
Mierzejewska, Jolanta;Chreptowicz, Karolina
Maf1蛋白是RNA聚合酶III的一般负抑制因子，在从酵母到人类的真核生物中都是保守的。在此，我们发现酵母maf1突变体增加了丙酮酸激酶活性，丙酮酸激酶是糖酵解的关键酶，也是在发酵和氧化代谢之间转换的重要参与者。我们观察到在葡萄糖上生长的maf1菌株乙醇产量增加，脂质含量升高。然而，在转向不可发酵的碳源后，观察到相反的效果，突变细胞积累了更小的脂滴。因此，我们认为Maf1蛋白对糖代谢和脂质稳态的调节至关重要。
The Maf1 protein is a general negative repressor of RNA polymerase III, which is conserved in eukaryotes from yeast to humans. Herein, we show the yeast maf1 mutant increases pyruvate kinase activity, the key enzyme in glycolysis and an important player in switching between fermentative and oxidative metabolism. We observed enhanced ethanol production and elevated lipid content in the maf1 strain grown on glucose. However, after shifting to a non-fermentable carbon source, the opposite effect was observed, and the mutant cells accumulated smaller lipid droplets. Thus, it has been concluded that the Maf1 protein is essential for regulation of glucose metabolism and lipid homeostasis.