Rickettsia felis:: molecular characterization of a new member of the spotted fever group
Rickettsia felis:: molecular characterization of a new member of the spotted fever group
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DOI：
10.1099/00207713-51-2-339
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发表时间：
2001-03-01
影响因子：
2.8
通讯作者：
Walker, DH
中科院分区：
文献类型：
--
作者：
Bouyer, DH;Stenos, J;Walker, DH
在这份报告中，放置猫立克次体斑点热组（SFG），而不是斑疹伤寒组（TC）的立克次体的建议。该生物体，这是第一次观察到猫蚤（猫栉首蚤）的电子显微镜，尚未被报道已培养可重复，从而限制了标准立克次体分型的血清学手段。为了克服这一挑战，选择了几个基因作为靶标用于R.猫来自自然感染了R.利用对190 kDa表面抗原（rOmpA）和17 kDa抗原基因特异的引物组通过PCR扩增猫。整个5513 bp的rompA基因进行测序，其特征在于，发现有几个独特的功能相比，其他立克次体的rompA基因。对17 kDa抗原基因部分序列的系统发育分析表明，该病毒与其它的红细胞膜抗原基因有很大的同源性.这些数据证实了以前报道的分析结果，即柠檬酸合酶，165 rRNA，rompB（135 kDa表面抗原），metK，ftsY，polA和dnaE基因，这些基因将R.猫蚤是SFC的成员。该微生物通过经阴道和经卵巢传播，在猫蚤的中肠、气管基质、肌肉、皮下组织、卵巢和睾丸中观察到感染。
In this report, placement of Rickettsia felis in the spotted fever group (SFG) rather than the typhus group (TC) of Rickettsia is proposed. The organism, which was first observed in cat fleas (Ctenocephalides felis) by electron microscopy, has not yet been reported to have been cultivated reproducibly, thereby limiting the standard rickettsial typing by serological means. To overcome this challenge, several genes were selected as targets to be utilized for the classification of R. felis. DNA from cat fleas naturally infected with R. felis was amplified by PCR utilizing primer sets specific for the 190 kDa surface antigen (rOmpA) and 17 kDa antigen genes. The entire 5513 bp rompA gene was sequenced, characterized and found to have several unique features when compared to the rompA genes of other Rickettsia. Phylogenetic analysis of the partial sequence of the 17 kDa antigen gene indicated that R. felis is less divergent from the SFG rickettsiae than from the TG rickettsiae, The data corroborate results from previous reports that analysed the citrate synthase, 165 rRNA, rompB (135 kDa surface antigen), metK, ftsY, polA and dnaE genes that placed R. felis as a member of the SFC. The organism is passed trans-stadially and transovarially, and infection in the cat flea has been observed in the midgut, tracheal matrix, muscle, hypodermis, ovaries and testes.