Inhibitor analysis of calf thymus DNA polymerases alpha, delta and epsilon.
Inhibitor analysis of calf thymus DNA polymerases alpha, delta and epsilon.
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DOI：
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
G. Wright;U. Hübscher;N. Khan;F. Focher;A. Verri
中科院分区：
文献类型：
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作者：
G. Wright;U. Hübscher;N. Khan;F. Focher;A. Verri
在类似的试验条件下，报告了小牛胸腺中复制型DNA聚合酶（pol）α、δ和β抑制剂的定量效应。羰基二磷酸盐是pols delta和epsilon的竞争性抑制剂，选择性是pols alpha的4至6倍。Aphidicolin抑制pols α和δ的选择性是pols α和δ的6- 10倍。“丁基苯基”核苷酸BuPdGTP和BuAdATP抑制pol α，与pol δ和pol β相比具有至少1000倍的选择性。在类似的测定条件下使用这些抑制剂允许区分三种酶。
Quantitative effects of inhibitors of the replicative DNA polymerases (pol) alpha, delta and epsilon from calf thymus are reported under similar assay conditions. Carbonyldiphosphonate was a competitive inhibitor of pols delta and epsilon, with 4- to 6-fold selectivity compared to pol alpha. Aphidicolin inhibited pols alpha and delta with 6- to 10-fold selectivity compared to pol epsilon. The 'butylphenyl' nucleotides, BuPdGTP and BuAdATP, inhibited pol alpha with at least 1000-fold selectivity compared to pols delta and epsilon. The use of these inhibitors under similar assay conditions permits the discrimination of the three enzymes.