Comparison of velocity vector imaging echocardiography with magnetic resonance imaging in mouse models of cardiomyopathy.
Comparison of velocity vector imaging echocardiography with magnetic resonance imaging in mouse models of cardiomyopathy.
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DOI：
10.1161/circimaging.111.972406
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Hoit BD
中科院分区：
文献类型：
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作者：
Azam S;Desjardins CL;Schluchter M;Liner A;Stelzer JE;Yu X;Hoit BD
使用超声心动图进行心肌应变成像是一种客观量化室壁运动的经济有效的方法，但很少有研究将超声心动图测量的应变与小动物MRI进行比较。我们在两种心肌病小鼠模型中比较了超声心动图（矢量速度成像[VVI]）测量的周向（CS）和径向应变（RS）与刺激回波（DENSE）MRI位移编码。在心肌肌球蛋白结合蛋白-C（cMyBP-C-/-，n=6）或肌肉LIM蛋白（MLP-/-，n=6）基因靶向缺陷的3月龄小鼠和野生型（WT）小鼠（n=8）中，在三个横截面水平测量心肌应变，并取平均值以获得全局应变。VVI和MRI测量的应变之间存在适度相关性，与全局RS相比，全局CS产生更强的相关性（CS R2 = 0.4452 vs. RS R2 = 0.2794，均p<0.05）。总体而言，通过VVI测量的应变比MRI更易变（p<0.05），全局CS的一致性限值略接近RS，但不显著（p=0.14）。VVI和MRI应变测量均显示与野生型相比，敲除组中的全局CS应变显著较低。两个基因敲除组的VVI（而非MRI）CS应变测量值不同（分别为−14.5 ± 3.8% vs. −6.6 ± 4.0%，cMyBP-C−/− vs. MLP−/−，p<0.05）。使用2D超声心动图在小动物中测量LV周向和径向应变是可行的。VVI和MRI应变测量值适度相关，并且环向应变的模式之间的一致性往往大于径向应变。虽然VVI和MRI品系能够区分野生型和基因敲除小鼠，但只有全局圆周VVI品系区分两种心肌病模型。
Myocardial strain imaging using echocardiography can be a cost-effective method to objectively quantify ventricular wall motion but few studies have compared strain measured with echocardiography against MRI in small animals. We compared circumferential (CS) and radial strain (RS) measured with echocardiography (vector velocity imaging [VVI]) to displacement encoding with stimulated-echo (DENSE) MRI in two mouse models of cardiomyopathy. In 3 month old mice with gene targeted deficiency of cardiac myosin binding protein-C (cMyBP-C−/−, n=6) or muscle LIM protein (MLP−/−, n=6), and wild-type (WT) mice (n=8), myocardial strains were measured at three cross-sectional levels and averaged to obtain global strains. There was modest correlation between VVI and MRI measured strains, with global CS yielding stronger correlation compared to global RS (CS R2 = 0.4452 vs. RS R2 = 0.2794, both p<0.05). Overall, strain measured by VVI was more variable than MRI (p<0.05) and the limits of agreement were slightly, but not significantly (p=0.14) closer for global CS than RS. Both VVI and MRI strain measurements showed significantly lower global CS strain in the knockout groups compared to the wild-type. The VVI (but not MRI) CS strain measurements were different between the two knockout groups (−14.5 ± 3.8% vs. −6.6 ± 4.0%, cMyBP-C−/− vs. MLP−/− respectively, p<0.05). Measurements of LV circumferential and radial strains are feasible in small animals using 2D echocardiography. VVI and MRI strain measurements correlated modestly and the agreement between the modalities tended to be greater for circumferential than radial strain. Although VVI and MRI strains were able to differentiate between wild-type and knockout mice, only global circumferential VVI strain differentiated between the two models of cardiomyopathy.