Understanding the Key Factors for Enzymatic Conversion of Pretreated Lignocellulose by Partial Least Square Analysis
Understanding the Key Factors for Enzymatic Conversion of Pretreated Lignocellulose by Partial Least Square Analysis
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通过偏最小二乘分析了解预处理木质纤维素酶促转化的关键因素
DOI：
10.1002/btpr.324
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发表时间：
2010-03-01
影响因子：
2.9
通讯作者：
He, Zhimin
中科院分区：
文献类型：
--
作者：
Huang, Renliang;Su, Rongxin;He, Zhimin
木质纤维素底物的理化性质与酶消化之间的关系尚不清楚。采用不同的预处理方法对纤维素酶进行水解，并通过柱溶质排斥、粒度分析、X射线衍射、傅里叶变换红外光谱和固态13C核磁共振等方法对纤维素酶的理化特性进行了测定。然后应用偏最小二乘法寻求限制纤维素消化速率和程度的关键因素。根据PLS结果，纤维素消化的最重要因素是可达的内表面积，其次是脱木质素和氢键的破坏。在2和24小时内，随着内表面积对直径为5.1 nm的报告分子的可接近性的增加，纤维素的消化得到改善。木质素的去除和氢键的断裂也能显著促进纤维素的转化。其他性质，包括分子内氢键的分解、纤维素的结晶度和半纤维素含量，对酶解效率的影响较小。©2009美国化学工程师学会生物技术。掠夺。, 2010年
The relationship between the physicochemical properties of lignocellulosic substrates and enzyme digestion is still not well known. After different pretreatments, cellulase hydrolysis and measurements of physicochemical characteristics by column solute exclusion, particle size analysis, X‐ray diffraction, Fourier transform infrared spectroscopy and solid state 13C nuclear magnetic resonance were performed in this study. Partial least squares was then applied to seek the key factors limiting the rate and extent of cellulose digestion. According to the PLS results, the most important factor for cellulose digestion was accessible interior surface area, followed by delignification and the destruction of the hydrogen bonds. The cellulose digestion at 2 and 24 hr were improved with the increased accessibility of interior surface area to the reporter molecules of 5.1‐nm diameter. Removal of lignin and breaking of hydrogen bonds were also found to significantly promote cellulose conversion. Other properties, including the breakdown of intramolecular hydrogen bonds, cellulose crystallinity, and hemicellulose content, had less effect on the efficiency of enzymatic hydrolysis. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010