Crystallographic studies of Mycobacterium tuberculosis polyphosphate/ATP-NAD kinase complexed with NAD
Crystallographic studies of Mycobacterium tuberculosis polyphosphate/ATP-NAD kinase complexed with NAD
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DOI：
10.1263/jbb.98.391
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发表时间：
2004-11-01
影响因子：
2.8
通讯作者：
Murata, K
中科院分区：
文献类型：
--
作者：
Mori, S;Yamasaki, M;Murata, K
来自结核分枝杆菌的NAD激酶（Ppnk）使用ATP或无机多磷酸盐[聚（P）]。纯化在大肠杆菌中过表达的Ppnk，并在NAD存在下结晶。对所得晶体的初步X射线分析表明，该晶体属于六方空间群P6（2）22，并与NAD形成全Ppnk络合物。
NAD kinase from Mycobacterium tuberculosis (Ppnk) uses ATP or inorganic polyphosphate [poly(P)]. Ppnk overexpressed in Escherichia coli was purified and crystallized in the presence of NAD. Preliminary X-ray analysis of the resultant crystal indicate that the crystal belongs to hexagonal space group P6(2)22 and is holo-Ppnk complexed with NAD.