Aneuploidy Affects Proliferation and Spontaneous Immortalization in Mammalian Cells
Aneuploidy Affects Proliferation and Spontaneous Immortalization in Mammalian Cells
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DOI：
10.1126/science.1160058
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发表时间：
2008-10-31
期刊：
影响因子：
56.9
通讯作者：
Amon, Angelika
中科院分区：
文献类型：
--
作者：
Williams, Bret R.;Prabhu, Vineet R.;Amon, Angelika
非整倍体，一种不正确的染色体数目，是人类流产和智力迟钝的主要原因，也是癌症的标志。我们通过产生一系列携带四条小鼠染色体之一的额外拷贝的细胞系来检查非整倍性对原代小鼠细胞的影响。在所有四个三体系中，增殖受损，代谢特性改变。永生化，即获得无限增殖的能力，也受到某些染色体额外拷贝的影响。我们的数据表明，非整倍体不仅降低了生物体，而且降低了不同非整倍体细胞之间共享的细胞适合度和弹性特征。
Aneuploidy, an incorrect number of chromosomes, is the leading cause of miscarriages and mental retardation in humans and is a hallmark of cancer. We examined the effects of aneuploidy on primary mouse cells by generating a series of cell lines that carry an extra copy of one of four mouse chromosomes. In all four trisomic lines, proliferation was impaired and metabolic properties were altered. Immortalization, the acquisition of the ability to proliferate indefinitely, was also affected by the presence of an additional copy of certain chromosomes. Our data indicate that aneuploidy decreases not only organismal but also cellular fitness and elicits traits that are shared between different aneuploid cells.