Identification of structural and morphogenesis genes of Pseudoalteromonas phage φRIO-1 and placement within the evolutionary history of Podoviridae.
Identification of structural and morphogenesis genes of Pseudoalteromonas phage φRIO-1 and placement within the evolutionary history of Podoviridae.
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DOI：
10.1016/j.virol.2015.12.005
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发表时间：
2016-02
期刊：
影响因子：
3.7
通讯作者：
Cho BC
中科院分区：
文献类型：
--
作者：
Hardies SC;Thomas JA;Black L;Weintraub ST;Hwang CY;Cho BC
通过质谱法和凝胶密度测定法对假交替单胞菌噬菌体 φRIO-1 的病毒体蛋白进行鉴定和定量。为处理极端分歧而定制的生物信息学方法定义了噬菌体的 φRIO-1 尾部结构同源组，该组进一步与 T7 尾部和内部病毒粒子蛋白（IVP）相关。类似地，除了小核病毒亚科之外，基本上所有已完全测序的足病毒中都鉴定出了管状尾部成分和内部病毒粒子蛋白的同源物。除 RIO-1 和 T7 组外，足病毒还被细分为几个尾部结构同源组。分子系统发育表明，这些群体都出现在与 φRIO-1/T7 分裂大致相同的古代时期。因此，涉及 IVP 的 T7 样感染机制是大多数足病毒的祖先特性。发现 IVP 不同程度地承载尾部溶菌酶结构域和去往细胞质的结构域，包括嵌入 IVP-D 同源物内的 N4 病毒体 RNA 聚合酶。
The virion proteins of Pseudoalteromonas phage φRIO-1 were identified and quantitated by mass spectrometry and gel densitometry. Bioinformatic methods customized to deal with extreme divergence defined a φRIO-1 tail structure homology group of phages, which was further related to T7 tail and internal virion proteins (IVPs). Similarly, homologs of tubular tail components and internal virion proteins were identified in essentially all completely sequenced podoviruses other than those in the subfamily Picovirinae. The podoviruses were subdivided into several tail structure homology groups, in addition to the RIO-1 and T7 groups. Molecular phylogeny indicated that these groups all arose about the same ancient time as the φRIO-1/T7 split. Hence, the T7-like infection mechanism involving the IVPs was an ancestral property of most podoviruses. The IVPs were found to variably host both tail lysozyme domains and domains destined for the cytoplasm, including the N4 virion RNA polymerase embedded within an IVP-D homolog.