EUKARYOTIC START AND STOP TRANSLATION SITES
EUKARYOTIC START AND STOP TRANSLATION SITES
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DOI：
10.1093/nar/19.12.3185
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发表时间：
1991-06-25
影响因子：
14.9
通讯作者：
RAY, SC
中科院分区：
文献类型：
--
作者：
CAVENER, DR;RAY, SC
翻译起始和终止位点侧翼的序列已被编译并统计分析了各种真核生物分类组。 分类群之间的一些关键相似之处支持保守的起始和终止机制。 然而，在不同真核生物群体内和之间的这些位点处的高度序列变异表明，许多mRNA的翻译可能受到调节。 二，三，和四核苷酸序列侧翼的起始/终止位点的多位置分析表明显着的偏见。 特别地，在起始密码子上游的-3、-2和-1位置处观察到强的三核苷酸偏倚。 这些偏见和种间变异的核苷酸偏好在这三个位置，使我们提出了一个修订后的模型的相互作用的18 S核糖体RNA与mRNA的翻译起始位点。 对终止密码子下游的CG二核苷酸的异常强烈的偏好表明它们可能导致错误的终止和/或核糖体与mRNA分离的失败。
Sequences flanking translational initiation and termination sites have been compiled and statistically analyzed for various eukaryotic taxonomic groups. A few key similarities between taxonomic groups support conserved mechanisms of initiation and termination. However, a high degree of sequence variation at these sites within and between various eukaryotic groups suggest that translation may be modulated for many mRNAs. Multipositional analysis of di-, tri-, and quadri-nucleotide sequences flanking start/stop sites indicate significant biases. In particular, strong tri-nucleotide biases are observed at the -3, -2, and -1 positions upstream of the start codon. These biases and the interspecific variation in nucleotide preferences at these three positions have lead us to propose a revised model of the interaction of the 18S ribosomal RNA with the mRNA at the site of translation initiation. Unusually strong biases against the CG dinucleotide immediately downstream of termination codons suggest that they may lead to faulty termination and/or failure of the ribosome to disassociate from the mRNA.