Pan‐cancer analysis of the oncogenic role of HNRNPR in human tumors
Pan‐cancer analysis of the oncogenic role of HNRNPR in human tumors
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DOI：
10.1002/cbin.12027
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发表时间：
2023-04
影响因子：
3.9
通讯作者：
Yi Yang;Junjun Sun;Zuo-Lin Xiang
中科院分区：
文献类型：
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作者：
Yi Yang;Junjun Sun;Zuo-Lin Xiang
异质核核糖核蛋白（hnRNP）是潜在的癌症生物标志物。HNRNPR是hnRNP家族的重要成员，对它在人类肿瘤中的作用知之甚少。本研究的目的是探索HNRNPR在癌症中的潜在价值，基于癌症基因组图谱（TCGA）。分析HNRNPR的表达水平、突变、DNA甲基化、磷酸化状态、生存状态、病理分期、肿瘤突变负荷（TMB）、微卫星不稳定性（MSI）、免疫细胞浸润和与HNRNPR相关的免疫特征。HNRNPR表达水平在几种类型的癌症中增加，并且与不良预后相关，特别是在肝细胞癌（LIHC）中。HNRNPR还与抗肿瘤免疫相关，并与TMB，MSI和癌症中的免疫细胞活化状态相关。根据HNRNPR和其他临床特征，建立了预测LIHC预后的列线图。功能富集分析显示了HNRNPR介导的LIHC进展的机制。功能丧失实验表明，抑制HNRNPR可显著抑制肝细胞癌（HCC）细胞增殖、迁移、侵袭和上皮-间质转化能力。我们的研究提供了对HNRNPR在不同肿瘤中的致癌作用的全面了解，并证明HNRNPR可能促进HCC细胞的增殖，迁移和侵袭能力。
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are potential cancer biomarkers. Little is known about the role of HNRNPR, an essential member of the hnRNP family, in human tumours. This study aims to explore the potential value of HNRNPR across cancers, based on The Cancer Genome Atlas (TCGA). The expression level, mutation, DNA methylation, phosphorylation status, survival status, pathological stage, tumor mutation burden (TMB), microsatellite instability (MSI), immune cell infiltration, and immune signature related to HNRNPR were analyzed. HNRNPR expression level was increased in several types of cancer and was associated with poor prognosis, especially in liver hepatocellular carcinoma (LIHC). HNRNPR was also correlated with antitumour immunity, and associated with TMB, MSI, and immune cell activation status across cancers. Furthermore, nomograms were established to predict the prognosis of LIHC, based on HNRNPR and other clinical characteristics. Functional enrichment analysis showed the mechanisms of HNRNPR‐mediated LIHC progression. Loss‐of‐function experiments demonstrated that inhibition of HNRNPR could remarkably suppress hepatocellular carcinoma (HCC) cell proliferation, migration, invasion, and Epithelial‐Mesenchymal Transition abilities. Our study offers a comprehensive understanding of the oncogenic roles of HNRNPR across different tumours, and demonstrates that HNRNPR might foster the proliferation, migration, and invasion abilities of HCC cells.