Protection from superoxide damage associated with an increased level of the YggX protein in Salmonella enterica
Protection from superoxide damage associated with an increased level of the YggX protein in Salmonella enterica
复制标题
DOI：
10.1073/pnas.151243198
复制
发表时间：
2001-07-03
影响因子：
11.1
通讯作者：
Downs, D
中科院分区：
文献类型：
--
作者：
Gralnick, J;Downs, D
超氧自由基对细胞生长和存活的有害影响主要是由蛋白质中不稳定的[Fe-S]簇的快速氧化引起的。这些簇的氧化释放出Fe（II）离子，参与破坏DNA的芬顿化学。本研究表明，YggX蛋白水平的升高增加了肠沙门氏菌对超氧胁迫的抵抗力，逆转了氧化[Fe-S]簇引起的酶缺陷，并降低了自发突变频率。该数据与YggX保护蛋白质[Fe-S]簇免于氧化的模型一致。
The deleterious effect of superoxide radicals on cell growth and survival is predominately caused by rapid oxidation of labile [Fe-S] clusters in proteins. Oxidation of these clusters releases Fe(II) ions, which participate in Fenton chemistry that damages DNA. Here it is shown that elevated levels of the YggX protein increase the resistance of Salmonella enterica to superoxide stress, reverse enzymatic defects attributed to oxidized [Fe-S] clusters, and decrease the spontaneous mutation frequency. The data are consistent with a model in which YggX protects protein [Fe-S] clusters from oxidation.