Aggregation of the scarab beetle Holotrichia consanguinea in response to female-released pheromone suggests secondary function hypothesis for semiochemical
Aggregation of the scarab beetle Holotrichia consanguinea in response to female-released pheromone suggests secondary function hypothesis for semiochemical
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DOI：
10.1007/bf02027731
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发表时间：
1996-08-01
影响因子：
2.3
通讯作者：
Vijayvergia, JN
中科院分区：
文献类型：
--
作者：
Leal, WS;Yadava, CPS;Vijayvergia, JN
用二氯甲烷和乙醚提取印度产农业害虫圣甲虫H. conanguinea雌甲虫的腹部腺体，并采用气相色谱-质谱法对其信息素系统进行了研究。用硅胶柱分离天然产物的室内生物测定表明，雄性对己烷-醚（80:20）分数有明显的性行为反应。虽然室内生物测定方法并没有鉴定出活性化合物，但对候选化学物质——苯甲醚、吲哚和苯酚的现场试验表明，甲虫对苯甲醚反应强烈。雄鼠和雌鼠在以茴香虫为诱饵的诱捕器中以接近1:1的比例捕获。在野外甲虫飞行活动期间捕获的甲虫中也观察到几乎相同的性别比例。因为没有明确的进化基础来解释为什么竞争的雌性会被这种符号化学物质吸引，所以它被简单地称为“雌性释放的信息素”。
The pheromone system of the scarab beetle H. consanguinea, an agricultural pest native to India, was investigated by extracting abdominal glands of females with dichloromethane and ether and analyzing them by GC-MS. Indoor bioassays with the natural product separated on a silica gel column showed that males responded to the hexane-ether (80:20) fraction by displaying a clear sexual behavior. Although the indoor bioassay-oriented approach did not lead to the identification of the active compound(s), field tests of candidate chemicals-anisole, indole, and phenol-showed that beetles responded strongly to anisole. Males and females were captured in anisole-baited traps in a ratio close to 1 : 1. Nearly the same sex ratio was also observed in beetles captured over the time during the flight activity of the beetles in the field. Because no clear evolutionary basis exists for why competing females are attracted to the semiochemical, it was simply referred to as a ''female-released pheromone.''