Architecture and dynamics of overlapped RNA regulatory networks
Architecture and dynamics of overlapped RNA regulatory networks
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DOI：
10.1261/rna.062687.117
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发表时间：
2017-11-01
期刊：
影响因子：
4.5
通讯作者：
Wickens, Marvin
中科院分区：
文献类型：
--
作者：
Lapointe, Christopher P.;Preston, Melanie A.;Wickens, Marvin
单个蛋白质可以结合并调节许多mRNA。具有相似特异性的多种蛋白质通常结合并控制重叠的mRNA组。然而，人们对重叠网络的结构或动力学知之甚少。我们重点研究了S.啤酒。使用RNA标签，我们确定了一个由四个子网络组成的“超级网络”：Puf 3 p，Puf 4p和Puf 5 p子网络，以及一个由Puf 4p和Puf 5 p控制的网络。单个子网络的架构，以及因此的超级网络，由特定PUF蛋白质之间结合mRNA的竞争、它们对结合元件的亲和力以及蛋白质的丰度决定。超级网络的反应是戏剧性的：剩余的网络可以扩展或收缩。这些截然不同的结果是由RNA和蛋白质的相对丰度及其相互亲和力之间的相互作用决定的。重叠的RNA-蛋白质网络之间的多样性相互作用为调节和进化提供了多方面的机会。
A single protein can bind and regulate many mRNAs. Multiple proteins with similar specificities often bind and control overlapping sets of mRNAs. Yet little is known about the architecture or dynamics of overlapped networks. We focused on three proteins with similar structures and related RNA-binding specificities-Puf3p, Puf4p, and Puf5p of S. cerevisiae. Using RNA Tagging, we identified a "super-network" comprised of four subnetworks: Puf3p, Puf4p, and Puf5p subnetworks, and one controlled by both Puf4p and Puf5p. The architecture of individual subnetworks, and thus the super-network, is determined by competition among particular PUF proteins to bind mRNAs, their affinities for binding elements, and the abundances of the proteins. The super-network responds dramatically: The remaining network can either expand or contract. These strikingly opposite outcomes are determined by an interplay between the relative abundance of the RNAs and proteins, and their affinities for one another. The diverse interplay between overlapping RNA-protein networks provides versatile opportunities for regulation and evolution.