Facile synthesis of Fe3O4@polyethyleneimine modified with 4-formylphenylboronic acid for the highly selective extraction of major catecholamines from human urine
Facile synthesis of Fe3O4@polyethyleneimine modified with 4-formylphenylboronic acid for the highly selective extraction of major catecholamines from human urine
复制标题
轻松合成经 4-甲酰基苯基硼酸修饰的 Fe3O4@聚乙烯亚胺，用于高选择性地从人尿中提取主要儿茶酚胺
DOI：
10.1002/jssc.201500377
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发表时间：
2015-08-01
影响因子：
3.1
通讯作者：
Xu, Guowang
中科院分区：
文献类型：
--
作者：
Qin, Qian;Li, Hua;Xu, Guowang
尿和血浆中的儿茶酚胺，特别是多巴胺、肾上腺素和去甲肾上腺素的水平已被用于辅助精神病的诊断和治疗。由于它们的低内源性浓度，测定三种主要的儿茶酚胺是非常困难的。硼酸盐吸附剂通常用于从复杂基质中提取这些顺式二醇化合物。本工作采用两步法合成了一种新型的4-甲酰基苯基硼酸修饰的磁性纳米粒子Fe 3 O 4 @PEI-FPBA。聚乙烯亚胺上丰富的氨基为硼酸酯配体提供了丰富的结合位点。在此，Fe 3 O 4 @PEI-FPBA对肾上腺素的吸附容量大大提高，其值为3.45 mg/g，这远大于不含聚乙烯亚胺的类似材料的吸附容量。磁性纳米粒子还表现出高的磁化强度（72.25 emu/g）和对儿茶酚胺的特定选择性。最后，将基于Fe 3 O 4 @PEI-FPBA纳米粒子的液相色谱-串联质谱法成功应用于人尿样中3种儿茶酚胺的测定。方法的线性、定量限、回收率和精密度均符合要求。结果表明，抑郁症患者的多巴胺、肾上腺素和去甲肾上腺素水平均高于正常人。
The levels of catecholamines, especially dopamine, epinephrine and norepinephrine in urine and plasma have been used to assist the diagnosis and treatment of psychosis. Due to their low endogenous concentrations, the determination of the three major catecholamines is very difficult. Boronate adsorbents are often employed to extract these cis-diol compounds from complex matrices. In this work, a novel type of magnetic nanoparticles modified with 4-formylphenylboronic named Fe3O4@PEI-FPBA was synthesized by a facile two-step approach. The abundant amino groups of polyethyleneimine provided the rich binding sites for boronate ligands. Herein, the adsorption capacity of Fe3O4@PEI-FPBA is greatly improved with a value of 3.45 mg/g towards epinephrine, which is much larger than that of analogous material without polyethyleneimine. The magnetic nanoparticles also exhibited high magnetization (72.25 emu/g) and specific selectivity towards the catecholamines. Finally, a liquid chromatography tandem mass spectrometry method based on Fe3O4@PEI-FPBA nanoparticles was successfully used to determine the three catecholamines from human urine samples. The linearity, limit of quantitation, recovery and precision of the method were satisfactory. Based on the method, it is found that the levels of dopamine, epinephrine and norepinephrine in depressive patients are higher than those in healthy controls.