Gut content identification of larvae of the Anopheles gambiae complex in western Kenya using a barcoding approach
Gut content identification of larvae of the Anopheles gambiae complex in western Kenya using a barcoding approach
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DOI：
10.1111/j.1471-8286.2007.02013.x
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发表时间：
2008-05-01
影响因子：
7.7
通讯作者：
Yan, G.
中科院分区：
文献类型：
--
作者：
Garros, C.;Ngugi, N.;Yan, G.
虽然幼虫取食和食物来源对非洲疟疾媒介的发展、生存和种群调节至关重要，但对冈比亚按蚊幼虫在自然环境中的捕食生物还没有得到很好的研究。本研究使用分子条形码方法研究冈比亚按蚊的天然食性。肯尼亚西部的幼虫。对自然栖息地的3龄和4龄幼虫的肠道内容物进行解剖并提取DNA。扩增18S核糖体DNA基因，用限制性片段长度多态方法筛选克隆，并对非蚊虫克隆进行测序。然后使用非蚊子克隆的序列进行同源性搜索和系统发育分析，以鉴定被摄取的推定微生物。系统发育分析将摄食的微生物聚在四个分支中，包括两个绿藻分支(绿藻门、绿藻纲、衣藻亚纲和绿球藻科)、一个真菌分支和一个未知的真核生物分支。同时，使用同样的方法，对幼虫栖息地的生物多样性进行了分析。本研究论证了条形码方法推断冈比亚按蚊幼虫天然食性的可行性。我们的分析表明，尽管自然栖息地中有广泛的微生物可用，但蚊子幼虫以特定的藻类为食。这项研究开发的新工具可用于提高我们对非洲疟疾媒介幼虫生态的了解，并促进开发新的蚊子控制工具。
Although larvae feeding and food source are vital to the development, survival and population regulation of African malaria vectors, the prey organisms of Anopheles gambiae larvae in the natural environment have not been well studied. This study used a molecular barcoding approach to investigate the natural diets of Anopheles gambiae s.l. larvae in western Kenya. Gut contents from third- and fourth-instar larvae from natural habitats were dissected and DNA was extracted. The 18S ribosomal DNA gene was amplified, the resulting clones were screened using a restriction fragment length polymorphism method and nonmosquito clones were sequenced. Homology search and phylogenetic analyses were then conducted using the sequences of non-mosquito clones to identify the putative microorganisms ingested. The phylogenetic analyses clustered ingested microorganisms in four clades, including two clades of green algae (Chlorophyta, Chlorophyceae Class, Chlamydomonadales and Chlorococcales families), one fungal clade, and one unknown eukaryote clade. In parallel, using the same approach, an analysis of the biodiversity present in the larval habitats was carried out. This present study demonstrated the feasibility of the barcoding approach to infer the natural diets of Anopheles gambiae larvae. Our analysis suggests that despite the wide range of microorganisms available in natural habitats, mosquito larvae fed on specific groups of algae. The novel tools developed from this study can be used to improve our understanding of the larval ecology of African malaria vectors and to facilitate the development of new mosquito control tools.