Lipopolysaccharides (LPS) modulate the metabolism of deoxynivalenol (DON) in the pig
Lipopolysaccharides (LPS) modulate the metabolism of deoxynivalenol (DON) in the pig
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DOI：
10.1007/s12550-014-0201-7
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发表时间：
2014-08-01
影响因子：
3
通讯作者：
Rothkoetter, Hermann-Josef
中科院分区：
文献类型：
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作者：
Daenicke, Sven;Valenta, Hana;Rothkoetter, Hermann-Josef
猪可能同时暴露于脂多糖（LPS）和脱氧雪腐镰刀菌烯醇（DON），并且这两种毒素被认为交互地影响肠道屏障、先天免疫系统和外源性物质代谢。因此，我们的目的是研究单一和两种毒素对营养物质消化率和DON代谢的综合影响。为此目的，对阉公猪（26 +/- 4 kg）限制性地饲喂对照日粮（CON）或被3.1 mg DON/kg（DON）污染的日粮37天。在实验的第37天，用100 μ g DON/kg体重（BW）（CON-DON）、7.5 μ g LPS/kg BW（CON-LPS、DON-LPS）或两种物质的组合（CON-DON+LPS）或生理盐水（CON-CON、DON-CON）静脉内输注猪60分钟。频繁采集血样，直至处死猪前3.25 h采集胆汁、肝脏和肾脏。无氮浸出物的表观消化率显着增加了1%，当喂食DON污染的饮食。当与单独输注DON的猪（18.8 ng/mL; CON-DON）相比时，血液中的总DON含量在内毒素血症猪（34.8 ng/mL; CON-DON+LPS）中显著更高，而胆汁浓度不受LPS的影响。肝脏和肾脏中的DON残留水平与血液中所述的治疗效果密切相关。与DON输注相反，当猪通过饮食暴露于DON时，LPS攻击导致显著较低的总DON浓度（在组DON-CON和DON-LPS中分别为13.2和7.5 ng/mL）。血液和胆汁中DON的结合程度不受处理的影响。总之，内毒素血症猪的特点是血液，肝脏和肾脏中的DON残留水平较高，可能是由于消除受损。
Pigs might be exposed to lipopolysaccharides (LPS) and deoxynivalenol (DON) at the same time, and both toxins are thought to interactively affect the intestinal barrier, the innate immune system, and the xenobiotics metabolism. Hence, we aimed at examining the single and combined effects of both toxins on nutrient digestibility and DON metabolism. For this purpose, barrows (26 +/- 4 kg) were fed restrictedly either a control diet (CON) or a diet contaminated with 3.1 mg DON/kg (DON) for 37 days. At day 37 of the experiment, pigs were infused intravenously for 60 min either with 100 mu g DON/kg body weight (BW) (CON-DON), 7.5 mu g LPS/kg BW(CON-LPS, DON-LPS) or a combination of both substances (CON-DON+LPS), or physiological saline (CON-CON, DON-CON). Blood samples were collected frequently until 3.25 h before the pigs were sacrificed for bile, liver, and kidney collection. The apparent digestibility of N-free extractives was significantly increased by 1 % when the DON-contaminated diet was fed. The total DON content in blood was significantly higher in endotoxemic pigs (34.8 ng/mL; CON-DON+LPS) when compared to the pigs infused with DON alone (18.8 ng/mL; CON-DON) while bile concentrations were not influenced by LPS. DON residue levels in liver and kidney closely reflected the treatment effects as described for blood. In contrast to DON infusion, the LPS challenge resulted in a significantly lower total DON concentration (13.2 vs. 7.5 ng/mL in groups DON-CON and DON-LPS, respectively) when the pigs were exposed to DON through the diet. The conjugation degree for DON in blood and bile was not influenced by treatments. In conclusion, endotoxemic pigs are characterized by higher DON residue levels in blood, liver, and kidney, probably by a compromised elimination.