Bioluminescence imaging of hepatitis B virus enhancer and promoter activities in mice
Bioluminescence imaging of hepatitis B virus enhancer and promoter activities in mice
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小鼠乙型肝炎病毒增强子和启动子活性的生物发光成像
DOI：
10.1016/j.febslet.2008.09.035
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发表时间：
2008-10-15
期刊：
影响因子：
3.5
通讯作者：
Zhan, Lin-Sheng
中科院分区：
文献类型：
--
作者：
Du, Juan;Zhou, Yong;Zhan, Lin-Sheng
采用生物发光成像技术和流体动力基因转移技术，研究了在真实生理条件下小鼠乙型肝炎病毒（HBV）启动子的活性以及HBV增强子对这些启动子的影响。我们的研究表明，两种HBV增强子均能刺激hepa 1-6细胞（体外）和小鼠肝脏（体内）中HBV主要启动子的活性，并且对三种启动子（S1， S2和X启动子）的增强作用在体内明显大于体外。这两种HBV增强剂在体内和体外对HBV启动子没有协同作用。(C) 2008年欧洲生化学会联合会。Elsevier b.v.版权所有。
By bioluminescence imaging and hydrodynamic gene transfer technology, the activities of hepatitis B virus (HBV) promoters and the effects of HBV enhancers on these promoters in mice under true physiological conditions have been assessed. Our studies reveal that either of the two HBV enhancers can stimulate HBV major promoter activity in hepa 1-6 cells (in vitro) and in mouse liver (in vivo), and the enhancer effects on the three promoters (S1, S2 and X promoter) are markedly greater in vivo than in vitro. The two HBV enhancers have no cooperative action on HBV promoters in vitro or in vivo. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.