The circadian system of Trypanosoma cruzi-infected mice
The circadian system of Trypanosoma cruzi-infected mice
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DOI：
10.1081/cbi-120017687
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发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Golombek, DA
中科院分区：
文献类型：
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作者：
Alfonso, TF;Celentano, AM;Golombek, DA
在克氏锥虫感染的C57-B16 J小鼠中研究了恰加斯病对哺乳动物昼夜节律系统的影响。动物接种CAI或RA菌株的克氏锥虫或车辆，通过血液标本可视化和运动活动节律分析轮运行记录证实寄生。RA株感染的小鼠表现出昼夜节律的幅度显着下降，无论是在光暗和恒定的黑暗条件下，可能是由于运动缺陷。CAI治疗的动物表现出正常的运动活动节律。然而，在这些小鼠中，再夹带到LD周期的6小时相移需要比对照组显著更长的时间，并且在DD中应用15分钟光脉冲产生了更小的节律相位延迟。各组大鼠视交叉上核均有光诱导的Fos表达。我们的结论是，克氏锥虫感染对昼夜节律系统的主要影响是从时钟到控制节奏的运动输出的损害，以及对昼夜视觉灵敏度的影响。
The effects of Chagas disease on the mammalian circadian system were studied in Trypanosoma cruzi-infected C57-B16J mice. Animals were inoculated with CAI or RA strains of T cruzi or vehicle, parasitism confirmed by blood specimen visualization and locomotor activity rhythms analyzed by wheel-running recording. RA-strain infected mice exhibited significantly decreased amplitude of circadian rhythms, both under light-dark and constant dark conditions, probably due to motor deficiencies. CAI-treated animals showed normal locomotor activity rhythms. However, in these mice, reentrainment to a 6h phase shift of the LD cycle took significantly longer than controls, and application of 15min light pulses in DD produced smaller phase delays of the rhythms. All groups exhibited light-induced Fos expression in the suprachiasmatic nuclei. We conclude that the main effect of T cruzi infection on the circadian system is an impairment of the motor output from the clock toward controlled rhythms, together with an effect on circadian visual sensitivity.