Studies in plaque pathogenicity. II. A technique for the specific detection of endotoxin in plaque samples using the limulus lysate assay.
Studies in plaque pathogenicity. II. A technique for the specific detection of endotoxin in plaque samples using the limulus lysate assay.
复制标题
斑块致病性的研究。
DOI：
10.1111/j.1600-0765.1978.tb00161.x
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发表时间：
1978
影响因子：
3.5
通讯作者：
H. Oshrain
中科院分区：
文献类型：
--
作者：
D. Fine;L. Tabak;A. Salkind;H. Oshrain
采用鲎试剂测定法（LLA）对松散粘附斑块（LAP）和粘附斑块（AP）的病理潜能进行了测定和比较。设计了一种在无热原条件下从龈上牙区和龈下牙区收集AP和LAP的技术。在受试者放弃清洁牙齿两周后，从牙齿（gi = < 1）上收集5个牙颌上样本（AP和LAP）。在不小于4mm且不大于7mm深度的牙周袋内放置一周后，从mylar条上收集5个龈下样本。所有的样品都被冻干，重组，并通过定量茚三酮反应分析斑块质量（Moore和Stein， 1954）。通过对Levin和Bang（1964）的LLA进行修改，对样品进行了鲎试剂活性分析。
A study was undertaken to assay and compare the pathological potential of loosely adherent plaque (LAP) with that of adherent plaque (AP) using the limulus lysate assay (LLA). A technique was devised for collection both AP And LAP from supra- as well as subgingival tooth areas under pyrogen-free conditions. Five supragingival samples (AP & LAP) were collected from teeth (G.I.= < 1) after subjects had abstained from tooth cleaning for two weeks. Five subgingival samples were collected from mylar strips one week after placement in periodontal pockets not less than 4 mm and not more than 7 mm in depth. All samples were lyophilized, recontituted and analyzed for plaque mass by the quantitative ninhydrin reaction (Moore and Stein, 1954). Samples were analyzed for limulus lysate activity by a modification of the LLA of Levin and Bang (1964).
Results from the five supragingival samples indicate that plaque mass, as determined by the ninhydrin reaction, was 4–5 fold greater in AP than in LAP. In the five subgingival samples, ninhydrin values for AP were two times higher than LAP. In spite of these differences in plaque mass, limulus activity in all LAP samples was consistently higher than in comparable AP samples when expressed as limulus activity per mole of amino acid. These preliminary findings suggest that subgingival LAP, situated in a position of strategic importance, is biologically active and deserves further study.